Anti-JNK2 Rabbit Polyclonal Antibody (KO Validated) for WB, ELISA - P45984

Core Product Specifications and Parameters

Biological Background: JNK2 (MAPK9) Function and Localization

• Mitogen-activated protein kinase 9 (MAPK9, JNK2) is a serine/threonine-protein kinase that regulates diverse cellular processes such as proliferation, differentiation, migration, transformation, and programmed cell death (PubMed:10376527, PubMed:15805466, PubMed:17525747). Related references: PMID:10376527 PMID:15805466 PMID:17525747

• JNK2 is a central component of the stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK) signaling pathway, activated by the dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 in response to pro-inflammatory cytokines and physical stress (PubMed:10376527, PubMed:15805466, PubMed:17525747). Related references: PMID:10376527 PMID:15805466 PMID:17525747

• Once activated, JNK2 phosphorylates transcription factors, notably JUN and ATF2, enhancing AP-1 transcriptional activity to mediate gene expression changes (PubMed:10376527). Related references: PMID:10376527

• JNK2 promotes apoptosis under stress conditions by phosphorylating TP53 and YAP1 (PubMed:17525747, PubMed:21364637). Related references: PMID:17525747 PMID:21364637

• The kinase also modulates circadian rhythm by phosphorylating the CLOCK-BMAL1 heterodimer (PubMed:22441692). Related references: PMID:22441692

• In T-cells, JNK2 (together with MAPK8) is required for Th1 polarization and is activated downstream of TCR signaling to regulate JUN levels (PubMed:19290929). Related references: PMID:19290929

• JNK2 localizes to both the cytoplasm and nucleus and is regulated by alternative splicing, producing isoforms (alpha‑1, alpha‑2, beta‑1, beta‑2) that differentially bind JUN and ATF2.

• JNK2 inhibits the canonical Wnt pathway by promoting beta-catenin degradation (PubMed:19675674) and disrupts tight junctions under osmotic stress (PubMed:20595622). Related references: PMID:19675674 PMID:20595622

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering the full-length human JNK2 (aa 1‑424), which may elicit polyclonal antibodies recognizing multiple epitopes across the protein. For Western blotting (WB), a band near 48 kDa is expected in human and mouse cell lysates; the use of KO‑validated lysates is recommended to confirm specificity.
• For ELISA applications, optimize antibody dilution and include appropriate positive (recombinant JNK2 protein or stimulated cell lysates) and negative controls. Cross‑reactivity has been assayed with human and mouse samples; evaluate reactivity in other species empirically.
• Given JNK2’s role in stress signaling, consider inducing stress pathways (e.g., UV, anisomycin) to observe activation‑dependent phosphorylation shifts if using phospho‑specific detection.

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