Anti-JNK1/2/3 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P45983 / P45984 / P53779

Core Product Specifications and Parameters

Biological Background: JNK1/2/3 Function and Localization

• MAPK8 (JNK1) is a serine/threonine-protein kinase that plays pivotal roles in cell proliferation, differentiation, migration, transformation, and programmed cell death. It is activated by extracellular stresses and pro-inflammatory cytokines through the SAP/JNK signaling pathway (PubMed:28943315). Related references: PMID:28943315
• Upon activation, JNK1 translocates to the nucleus and phosphorylates multiple transcription factors, particularly AP-1 components such as c-Jun, JDP2, and ATF2, thereby modulating AP-1 transcriptional activity (PubMed:18307971). Related references: PMID:18307971
• JNK1 promotes apoptosis in stressed cells by directly phosphorylating key regulators p53/TP53 and Yes-associated protein YAP1 (PubMed:21364637). Related references: PMID:21364637
• It mediates starvation-induced autophagy by phosphorylating BCL2, leading to its dissociation from BECN1 and activation of the autophagic machinery (PubMed:18570871). Related references: PMID:18570871
• JNK1 also participates in circadian rhythm regulation by phosphorylating the CLOCK-BMAL1 heterodimer (PubMed:22441692). Related references: PMID:22441692
• Alternative splicing generates JNK1 isoforms that display differential binding to c-Jun and ATF2, although all isoforms phosphorylate these targets with similar efficiency.
• The protein localizes to the cytoplasm, nucleus, and synaptic compartments, consistent with its roles in both nuclear transcription regulation and cytoplasmic signaling.

Experimental Guidance and Technical Tips

• The immunogen peptide sequence resides within a conserved region of JNK1 (amino acids 200-300), which likely accounts for the observed cross-reactivity with mouse and rat JNK proteins.
• For Western blotting, expect bands around 35, 44, and 48 kDa representing JNK1/2/3 isoforms; validation in relevant tissue lysates or stimulated cell lines is recommended.
• In immunofluorescence/ICC, consider that JNK shuttles between cytoplasm and nucleus upon activation; optimize permeabilization and inclusion of activation controls.
• For ELISA applications, this antibody may be used as a capture or detection reagent; pairing with validated matched antibody pairs can enhance assay sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers and diagnostic developers studying stress kinase signaling and related pathways, CamelBio provides one-stop sourcing for high-quality JNK antibodies and associated reagents, supporting your journey from concept to clinic. Our expertise in rare target raw-material sourcing ensures you have reliable access to the tools you need.