Anti-JNK1/2/3 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P45983 / P45984 / P53779

Core Product Specifications and Parameters

Biological Background: JNK1/2/3 Function and Localization

• Belongs to the mitogen-activated protein kinase (MAPK) superfamily and is officially designated MAPK8 (JNK1), MAPK9 (JNK2), and MAPK10 (JNK3). This antibody recognizes all three JNK kinases based on its immunogen design.
• Functions as a stress-activated serine/threonine-protein kinase that phosphorylates transcription factors such as JUN, ATF2, and JDP2, thereby regulating AP-1 transcriptional activity (PubMed:18307971). Related references: PMID:18307971
• Plays a central role in apoptosis by phosphorylating p53/TP53 and YAP1, and modulates autophagy through BCL2 phosphorylation and dissociation from BECN1 (PubMed:21364637, PubMed:18570871). Related references: PMID:21364637 | PMID:18570871
• Regulates DNA replication by phosphorylating CDT1, which disrupts CDT1-HBO1 interaction and inhibits origin licensing (PubMed:21856198). Related references: PMID:21856198
• Localizes to the cytoplasm, nucleus, and synapse, and is involved in neuronal functions such as neurite elongation and synaptic vesicle capture (By similarity).
• Participates in immune and stress responses, including NLRP3 inflammasome assembly (PubMed:28943315) and regulation of heat shock factor HSF1 (PubMed:10747973). Related references: PMID:28943315 | PMID:10747973
• Contributes to circadian rhythm regulation by phosphorylating the CLOCK-BMAL1 heterodimer (PubMed:22441692). Related references: PMID:22441692
• Multiple isoforms exist due to alternative splicing; isoforms display differential binding affinities for c-Jun and ATF2, though phosphorylation efficiency is similar.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-383 of human JNK1 (P45983), covering the full-length protein except the C‑terminal tail; this region is highly conserved among JNK1/2/3, supporting expected reactivity with all three kinases.
• For Western blot, expect bands around 46 kDa and 54 kDa due to multiple isoforms and phosphorylation states; validate in the relevant sample system with appropriate controls.
• In immunofluorescence-paraffin (IF-P) applications, optimal antigen retrieval and permeabilization conditions should be determined empirically for each tissue type.
• ELISA performance may depend on assay format (direct, indirect, or sandwich); consider using the antibody as a capture or detection reagent after proper validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies high-performance IVD raw materials to diagnostic developers and research laboratories worldwide. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents for the MAPK signaling cascade. We support everything from early-stage assay development to scaled-up manufacturing, offering reliable bulk sourcing of rare-target antibodies like JNK1/2/3 to accelerate your timelines and reduce vendor complexity.