Anti-JNK1 Rabbit Polyclonal Antibody for WB, IF, IHC, ELISA - P45983

Core Product Specifications and Parameters

Biological Background: JNK1 Function and Localization

• Mitogen-activated protein kinase 8 (JNK1), encoded by the MAPK8 gene, is a stress-activated serine/threonine kinase that functions as a key component of the c-Jun N-terminal kinase (JNK) signaling cascade. It is also known as SAPK1c and JNK-46.
• Upon activation by dual phosphorylation from upstream kinases MAP2K4/MKK4 and MAP2K7/MKK7, JNK1 translocates to the nucleus and phosphorylates transcription factors such as c-Jun and ATF2, thereby regulating AP-1-dependent gene expression. Related references: PMID:18307971
• JNK1 promotes stress-induced apoptosis by phosphorylating key proteins including p53 and YAP1, linking cellular stress to programmed cell death. Related references: PMID:21364637
• Under starvation conditions, JNK1 phosphorylates BCL2, causing its dissociation from BECN1 and subsequent activation of autophagy. Related references: PMID:18570871
• The JNK1 gene undergoes alternative splicing to produce multiple isoforms (alpha-1, alpha-2, beta-1, beta-2) that exhibit different binding preferences for downstream substrates; for instance, beta-1 preferentially binds c-Jun, while alpha and beta-2 show similar low-level binding to both c-Jun and ATF2.
• JNK1 localizes predominantly to the cytoplasm and nucleus, and has also been found at synapses in neuronal cells, consistent with its roles in neurodevelopment and synaptic function.
• JNK1 participates in diverse physiological processes including circadian rhythm regulation by phosphorylating the CLOCK-BMAL1 complex, T-cell differentiation, and neuronal migration. Related references: PMID:22441692

Experimental Guidance and Technical Tips

• The immunogen was designed against residues 245–345 within the kinase domain of human JNK1, a region highly conserved among species, which supports the reported cross-reactivity with mouse and rat. Researchers may consider verifying reactivity in their specific model system.
• For Western blotting, a predominant band around 48 kDa is expected. Include appropriate positive and negative controls; whole-cell lysates from stressed or stimulated cells (e.g., UV-treated HeLa) may enhance signal due to activation-induced stabilization.
• In immunocytochemistry/immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), both nuclear and cytoplasmic staining patterns are anticipated. For neuronal tissues, synaptic localization may also be observed. Optimize fixation and permeabilization conditions for each sample type.
• Because this antibody recognizes total JNK1, detection of the activated (phosphorylated) form requires separate phospho-specific antibodies. It is recommended to run parallel blots or multiplex staining to distinguish activation states.
• For ELISA applications, consider using the antibody as a capture or detection reagent against recombinant JNK1 or cell lysates, pairing with a non-competing antibody for sandwich formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material partner, offering a seamless supply chain from discovery to commercial scale. Our catalog includes validated antibody pairs, bulk ancillary reagents, and access to rare-target materials, all backed by expert technical consultation. With this anti-JNK1 polyclonal antibody, you gain a reliable tool for stress-signaling biomarker assays, and our team is ready to assist with custom sourcing for your entire diagnostic development pipeline.