Anti-JNK1 Rabbit Monoclonal Antibody [KD Validated] for WB, IP, ELISA - P45983

Core Product Specifications and Parameters

Biological Background: JNK1 Function and Localization

• Mitogen-activated protein kinase 8 (MAPK8/JNK1) is a serine/threonine-protein kinase central to the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway, activated by pro-inflammatory cytokines and physical stress.
  Related references: PMID:28943315

• JNK1 phosphorylates key transcription factors including c-Jun, JDP2, and ATF2, thereby modulating AP-1 transcriptional activity and downstream gene expression.
  Related references: PMID:18307971

• The kinase promotes apoptosis via phosphorylation of p53/TP53 and YAP1, while also supporting erythroid cell survival through BAD phosphorylation upon EPO stimulation.
  Related references: PMID:21364637 PMID:21095239

• JNK1 regulates autophagy by mediating starvation-induced BCL2 phosphorylation, leading to BECN1 dissociation and autophagy activation.
  Related references: PMID:18570871

• It participates in circadian rhythm control by phosphorylating the CLOCK-BMAL1 heterodimer and contributes to NLRP3 inflammasome assembly.
  Related references: PMID:22441692 PMID:28943315

• Multiple JNK1 isoforms (alpha-1, alpha-2, beta-1, beta-2) exist with differential substrate binding preferences; beta-1 preferentially binds c-Jun, while all isoforms phosphorylate substrates with similar efficiency.

• Subcellular localization encompasses the cytoplasm, nucleus, and synapse, consistent with its roles in signal transduction, transcriptional regulation, and neuronal function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 250–350 within the kinase domain; the antibody may recognize multiple JNK1 isoforms. Use isoform-specific controls if needed.
• Before critical experiments, validate the antibody's specificity in your sample system, e.g., by JNK1 knock-down or knock-out samples.
• For Western blotting, a band near 48 kDa is expected. Activation by stress or cytokines may alter phosphorylation-dependent mobility; include appropriate lysate preparation controls.
• The antibody cross-reacts with mouse and rat JNK1, making it suitable for studies in common experimental models; ensure cross-reactivity is verified under your exact conditions.
• For IP and ELISA, titrate the antibody concentration under your reaction conditions to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to essential IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this JNK1 rabbit mAb. Beyond this product, we supply bulk ancillary reagents, complementary detection antibodies, and can source rare-target raw materials to support your complete assay development pipeline. Partner with CamelBio for reliable, one-stop procurement that bridges your concept to clinic.