Anti-JNK1 Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P45983

Core Product Specifications and Parameters

Biological Background: JNK1 Function and Localization

• Encoded by MAPK8, JNK1 (SAPK1c, JNK-46) is a serine/threonine-protein kinase of the MAP kinase family.
• Activated by upstream kinases MAP2K4/MKK4 and MAP2K7/MKK7 in response to stress stimuli, it phosphorylates c-Jun and ATF2 to regulate AP-1-driven transcription. Related references: PMID:18307971
• Promotes apoptosis by phosphorylating p53/TP53 and YAP1. Related references: PMID:21364637
• Mediates autophagy through starvation-induced BCL2 phosphorylation and BECN1 dissociation. Related references: PMID:18570871
• Phosphorylates additional substrates such as HSF4, SIRT1, ELK1, and ITCH, contributing to heat shock response, deacetylation, and ubiquitination. Related references: PMID:16581800, PMID:17296730, PMID:20027304
• Localizes to cytoplasm, nucleus, and synapse, enabling regulation of nuclear transcription and synaptic functions.
• Plays a role in circadian clock regulation by phosphorylating CLOCK-BMAL1. Related references: PMID:22441692

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 200-300 within the kinase domain; thus, the antibody may detect multiple JNK1 isoforms and possibly other JNK family members. Verify specificity with knockout controls or isoform-specific assays.
• For Western blot, positive control lysates from cells treated with anisomycin or UV can show activation-dependent phosphorylation shifts. The predicted molecular weight is 48 kDa, but post-translational modifications may alter migration.
• For immunohistochemistry (IHC-P and IF-P), optimize antigen retrieval and antibody dilution; validate with blocking peptide or tissue from MAPK8 knockout models.
• In ELISA applications, consider coating recombinant JNK1 protein or peptide and titrate the antibody for optimal sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio provides this rabbit monoclonal anti-JNK1 antibody for diagnostic and research applications in stress kinase signaling. Our portfolio includes validated antibody pairs, bulk ancillary reagents, and custom conjugation services to support assay development. We also source rare targets and recombinant proteins, accelerating your path from concept to clinic.