Anti-JMJD8 Polyclonal Antibody for WB, ELISA - Q96S16

Core Product Specifications and Parameters

Biological Background: JMJD8 Function and Localization

• JMJD8 (also known as PP14397 or C16orf20) is a JmjC domain-containing protein, belonging to the Jumonji family, encoded by the JMJD8 gene in humans.
• Functions as a positive regulator of TNF-induced NF-kappa-B signaling. Related references: PMID:27671354
• Regulates angiogenesis and cellular metabolism through interaction with pyruvate kinase M2 (PKM). Related references: PMID:27199445
• Subcellular localization includes the endoplasmic reticulum lumen and the cytoplasm, consistent with a signal peptide for secretion or membrane association.
• Annotated as a glycoprotein, indicating post-translational modifications that may affect its function and localization.
• Undergoes alternative splicing, potentially generating multiple protein isoforms with distinct properties.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length protein (amino acids 1-285), which may offer broad isoform coverage; however, validate detection of specific isoforms in your system.
• For Western blotting, consider starting with a dilution series to determine optimal working conditions for your sample type.
• The antibody is suitable for ELISA; ensure the target protein is present in your sample matrix and use appropriate controls.
• Reported cross-reactivity with human, mouse, and rat proteins; verify performance in your species and tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from early development to clinical application. With expertise in NF-kB signaling and metabolic targets like JMJD8, we provide validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development and manufacturing needs.