Anti-JMJD7 Polyclonal Antibody for WB, ELISA - P0C870

Core Product Specifications and Parameters

Biological Background: JMJD7 Function and Localization

• JMJD7 is a bifunctional enzyme possessing both endopeptidase and 2-oxoglutarate-dependent monooxygenase activities. Related references: PMID:28847961 PMID:29915238
• It cleaves the N-terminal tails of histones H2, H3, and H4 at the carboxyl side of methylated arginine or lysine residues, generating 'tailless nucleosomes' that may facilitate transcription elongation. Related references: PMID:28847961
• JMJD7 preferentially recognizes and cleaves monomethylated and dimethylated arginine residues, and can further digest histone tails via its aminopeptidase activity. Related references: PMID:28847961
• As a (3S)-lysyl hydroxylase, JMJD7 catalyzes Fe(2+) and 2-oxoglutarate-dependent stereospecific hydroxylation of 'Lys-22' in DRG1 and 'Lys-21' in DRG2 translation factors, potentially modulating their interaction with ribonucleic acids. Related references: PMID:29915238
• The protein localizes to both the nucleus and cytoplasm, consistent with its dual roles in chromatin modification and translation regulation.
• Structurally, JMJD7 contains a JmjC domain, a disulfide bond, and metal-binding sites, and is classified under hydrolases, oxidoreductases, and proteases.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the full-length human JMJD7 (aa 1-316). Due to high sequence homology among human, mouse, and rat, this antibody is expected to cross-react with these species, but independent validation is recommended.
• For Western blotting, a specific band around 36 kDa is anticipated. Use appropriate nuclear and cytoplasmic extraction protocols to detect JMJD7 in both compartments.
• ELISA performance may vary depending on coating antigen and detection format. Optimization of coating concentration and antibody dilution is advised.
• Consider including appropriate controls (e.g., recombinant protein, knockout cell lysates) to confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing high-quality antibodies like this JMJD7 polyclonal antibody to support research in chromatin biology and protein translation. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For unique or challenging targets, our team assists in rare target raw-material sourcing, helping to accelerate your diagnostic development from concept to clinic.