Anti-JMJD6 Polyclonal Antibody for WB, IHC-P, ELISA - Q6NYC1

Core Product Specifications and Parameters

Biological Background: JMJD6 Function and Localization

• JMJD6 is a bifunctional enzyme that acts as both an arginine demethylase and a lysyl-hydroxylase, belonging to the JmjC domain-containing protein family. Related references: PMID:17947579 PMID:20684070 PMID:21060799
• As a lysyl-hydroxylase, JMJD6 catalyzes 5-hydroxylation of specific lysine residues on proteins like U2AF2/U2AF65 and LUC7L2, thereby regulating pre-mRNA splicing. Related references: PMID:19574390
• JMJD6 plays a role in nucleolar liquid-liquid phase separation (LLPS) by post-translationally modifying LIAT1 at its lysine-rich domain, which inhibits LIAT1 nucleolar targeting.
• It acts as an arginine demethylase that preferentially removes asymmetric dimethylation marks from histones H3 (Arg-2) and H4 (Arg-3), contributing to histone code regulation and transcriptional activation. Related references: PMID:17947579 PMID:24360279 PMID:24498420
• In collaboration with BRD4, JMJD6 interacts with the P-TEFb complex to regulate polymerase II promoter-proximal pause release, enhancing transcription of target genes. It demethylates H4R3me2 and the methyl cap of 7SK snRNA to dismiss repressive complexes. Related references: PMID:24360279
• JMJD6 is primarily localized in the nucleus (nucleoplasm and nucleolus) but can also be found in the cytoplasm. It is highly expressed in heart, skeletal muscle, and kidney, with lower levels in other tissues, and is upregulated in chronic pancreatitis.
• The protein contains key domains for metal binding and dioxygenase activity, and is subject to alternative splicing and phosphorylation, underscoring its regulatory roles in development, differentiation, and mRNA processing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-170) of human JMJD6. This region may be conserved across species, supporting the observed cross-reactivity with mouse.
• As a polyclonal antibody, it may recognize multiple isoforms or post-translationally modified forms of JMJD6. Consider validating the detection of specific bands in WB in the relevant sample system.
• Predicted molecular weight of JMJD6 is approximately 46 kDa. Confirm band size with appropriate controls; additional bands may appear due to alternative splicing or modifications.
• For IHC-P, optimize antigen retrieval and antibody dilution using tissues known to express JMJD6 (e.g., heart, skeletal muscle, kidney).
• This antibody is suitable for ELISA; consider pairing with a matched detection antibody for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with reliable access to IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-JMJD6 polyclonal antibody exemplifies our commitment to sourcing critical targets in epigenetic regulation and RNA biology. Whether you need rare-target antibodies or complete raw-material packages for assay development, CamelBio streamlines procurement from concept to clinic.