Anti-JMJD4 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H9V9

Core Product Specifications and Parameters

Biological Background: JMJD4 Function and Localization

• JMJD4, also known as JmjC domain-containing protein 4, Jumonji domain-containing protein 4, or lysyl-hydroxylase JMJD4, is a 2-oxoglutarate and iron-dependent oxygenase belonging to the JmjC domain-containing protein family.
• It catalyzes the C4-lysyl hydroxylation of eukaryotic peptide chain release factor subunit 1 (ETF1) at Lys-63, a key post-translational modification that enhances the efficiency of translational termination.
• Subcellularly, JMJD4 localizes predominantly to the cytoplasm, consistent with its role in modifying a cytoplasmic translation factor.
• The protein is subject to alternative initiation and alternative splicing, yielding multiple isoforms that may have distinct regulatory properties.
• As a dioxygenase, JMJD4 requires iron as a cofactor and functions as an oxidoreductase, linking it to broader cellular oxygen-sensing and metabolic pathways.
• It has been identified in proteomics studies and is part of the human reference proteome.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody covers the full-length human JMJD4 protein (amino acids 1–463), which is expected to recognize all isoforms of JMJD4.
• For Western blotting, the predicted molecular weight of JMJD4 is approximately 47 kDa (UniProt), but the observed band may migrate around 52 kDa; pre-stained markers and appropriate controls are recommended.
• In immunofluorescence/ICC, JMJD4 signals should be localized to the cytoplasm; subcellular fractionation or co-staining with compartment-specific markers can help confirm specificity.
• This antibody is validated for ELISA; when setting up sandwich assays, consider pairing with a monoclonal antibody specific to a different epitope to minimize cross-reactivity.
• Since JMJD4 is conserved across human, mouse, and rat, experimental results from one species may be broadly transferable, but always verify cross-reactivity in your specific sample matrix.
• For novel applications (e.g., IP, ChIP), perform preliminary titration and validation experiments as reactivity in these contexts has not been characterized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers, research laboratories, and academic institutes, offering one-stop access to IVD raw materials, technical services, and consulting from concept to clinic. This anti-JMJD4 polyclonal antibody supports studies in protein modification and translational control, and we can assist with related needs such as validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. Whether you require scaling up, customization, or complementary reagents, CamelBio streamlines your supply chain so you can focus on advancing your research and diagnostic development.