Anti-JMJD1C Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q15652

Core Product Specifications and Parameters

Biological Background: JMJD1C Function and Localization

• JMJD1C (Jumonji domain-containing protein 1C), also known as KDM3C, TRIP8, and JHDM2C, is a member of the JMJD1/KDM3 family of lysine demethylases.
• It catalyzes the demethylation of lysine residues on non-histone substrates, such as STAT3 and MDC1, regulating key cellular processes. Demethylation of MDC1 promotes its interaction with RNF8 and facilitates RNF8-dependent ubiquitination, essential for DNA double-strand break repair. Related references: PMID:24240613
• The enzymatic activity of JMJD1C toward histone proteins remains debated; while it may act as a context-specific histone demethylase (e.g., H3K9me2 demethylation at lipogenic gene promoters), it may also function as a scaffold that recruits other active demethylase complexes. Related references: PMID:23593242 PMID:24240613 PMID:32034158
• In the liver, JMJD1C is recruited by USF1 to lipogenic promoters in response to insulin or feeding, potentially enhancing chromatin accessibility and lipogenic gene transcription. Related references: PMID:32034158
• JMJD1C localizes to the nucleus and functions as a coactivator of the androgen receptor, playing an indispensable role in spermatogenesis.
• The protein contains zinc-finger domains and belongs to the dioxygenase superfamily, requiring iron as a cofactor for its demethylase activity. Post-translational modifications, such as phosphorylation and ubiquitination, modulate its function.
• With a molecular weight of approximately 285 kDa, JMJD1C is a large multi-domain chromatin regulator implicated in transcription regulation and DNA repair.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 354–448 of human JMJD1C, an N‑terminal region distinct from the catalytic Jumonji domain. Researchers should consider this when designing domain-specific detection or functional studies.
• For Western blotting, JMJD1C migrates at approximately 285 kDa. Use a low‑percentage SDS‑PAGE gel (e.g., 6–8%) and extended transfer times to ensure efficient transfer of this high‑molecular‑weight protein.
• In immunohistochemistry (IHC‑P), signal may be nuclear. Include appropriate tissue controls and validate staining in the relevant species (human, mouse, or rat).
• ELISA applications can be developed for quantitative detection; optimal coating and detection conditions should be determined experimentally.

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CamelBio provides diagnostic manufacturers and researchers with one-stop access to high-quality IVD raw materials, including this anti-JMJD1C polyclonal antibody for chromatin regulation and DNA repair studies. Our portfolio extends to validated antibody pairs, bulk ancillary reagents, and custom sourcing of rare-target antibodies, supporting your assay development from research to clinical application.