Anti-JARID2 Rabbit Polyclonal Antibody for WB and ELISA - Q92833

Core Product Specifications and Parameters

Biological Background: JARID2 Function and Localization

• JARID2, also known as Jumonji/ARID domain-containing protein 2, is a 139 kDa nuclear protein belonging to the Jumonji family, but it lacks histone demethylase activity.
• It functions as a regulator of histone methyltransferase complexes, playing an essential role in embryonic development, including heart and liver development, neural tube fusion, and hematopoiesis. Related references: PMID:20075857
• Acts as an accessory subunit of the PRC2 (Polycomb repressive complex 2) complex, which mediates histone H3K27 trimethylation (H3K27me3) on chromatin, a repressive mark critical for gene silencing. Related references: PMID:20075857, PMID:29499137, PMID:31959557
• Binds DNA and facilitates the recruitment of PRC2 to target genes in embryonic stem cells, controlling stem cell differentiation and normal development. Related references: PMID:20075857
• In cardiac cells, JARID2 represses cyclin-D1 (CCND1) expression by promoting histone H3K9 methylation by GLP1/EHMT1 and G9a/EHMT2 methyltransferases.
• It also acts as a transcriptional repressor of ANF through interaction with transcription factors GATA4 and NKX2-5, and participates in negative regulation of cell proliferation.
• During embryogenesis, JARID2 is predominantly expressed in neurons, particularly in dorsal root ganglion cells, consistent with its developmental roles.
• Keywords: chromatin regulator, developmental protein, alternative splicing, acetylation, phosphoprotein, intellectual disability, nucleus.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1027-1246 of human JARID2, a region likely to be exposed and immunogenic; consider this when designing epitope-specific detection.
• For Western blot, a band around 139 kDa is expected. Use appropriate gel percentage and transfer conditions for high molecular weight proteins. Validate detection in nuclear extracts due to predominant nuclear localization.
• The antibody is reactive with human, mouse, and rat samples; therefore, it should be suitable for cross-species studies. However, always verify reactivity in your specific samples.
• For ELISA, consider using a standard recombinant protein or peptide corresponding to the immunogen region for assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a trusted partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your assay development. For JARID2 and other chromatin/epigenetics targets, we can source rare raw materials and deliver customized solutions, helping you accelerate your research and IVD kit production.