Anti-JARID2 Rabbit Monoclonal Antibody for WB, ELISA - Q92833

Core Product Specifications and Parameters

Biological Background: JARID2 Function and Localization

• JARID2 is a Jumonji/ARID domain-containing protein 2 (also known as JMJ, DIDDF), which functions as a chromatin regulator critical for embryonic development.
• Functions as an accessory subunit of the Polycomb Repressive Complex 2 (PRC2), mediating histone H3 lysine 27 trimethylation (H3K27me3) and recruiting PRC2 to target genes in embryonic stem cells (PubMed:20075857, PubMed:29499137, PubMed:31959557). Related references: PMID:20075857, PMID:29499137, PMID:31959557
• Plays essential roles in heart, liver, and neural tube development, as well as hematopoiesis (PubMed:20075857). Related references: PMID:20075857
• In cardiac cells, represses cyclin D1 (CCND1) expression via H3K9 methylation by GLP1/EHMT1 and G9a/EHMT2, and represses ANF through interaction with GATA4 and NKX2-5.
• Negatively regulates cell proliferation signaling and does not possess intrinsic histone demethylase activity.
• Localizes to the nucleus and is predominantly expressed in neurons, particularly dorsal root ganglion cells, during embryogenesis.
• Post-translationally modified by acetylation and phosphorylation; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blotting, a specific band is expected at approximately 139 kDa. Optimize antibody dilution and validate with appropriate positive and negative control lysates.
• In ELISA, peptide competition assays can be used to confirm binding specificity; titration of both coating antigen and antibody is recommended.
• Cross-reactivity with mouse and rat orthologs is indicated; validate performance in the relevant species and tissue type.
• Consider using knockout or knockdown models to confirm antibody specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material sourcing, from validated antibody pairs and monoclonal antibodies to bulk ancillary reagents. For your chromatin regulation and developmental biology research needs, we offer one-stop access to high-quality antibodies against JARID2 and other key targets, streamlining your path from concept to clinic.