Anti-JARID1B Polyclonal Antibody for WB, ELISA - Q9UGL1

Core Product Specifications and Parameters

Biological Background: JARID1B Function and Localization

• JARID1B/KDM5B is a histone lysine demethylase that specifically demethylates mono-, di-, and trimethylated lysine 4 of histone H3 (H3K4me1/me2/me3), playing a central role in epigenetic regulation and the histone code. Related references: PMID:24952722 PMID:27214403 PMID:28262558
• It does not exhibit demethylase activity toward H3K9 or H3K27 methylation marks.
• JARID1B acts as a transcriptional co-repressor by interacting with transcription factors such as FOXG1B and PAX9, modulating gene expression programs.
• In breast cancer, JARID1B promotes cell proliferation by repressing tumor suppressor genes including BRCA1 and HOXA5. Related references: PMID:24952722
• Conversely, in melanoma, JARID1B may function as a tumor suppressor, highlighting context-dependent roles in cancer.
• JARID1B is a core component of the circadian clock machinery, repressing CLOCK-BMAL1-mediated transcriptional activation of the PER2 gene.
• The protein localizes predominantly to the nucleus, consistent with its role in chromatin modification.
• JARID1B is a Fe(II) and α-ketoglutarate-dependent dioxygenase that contains multiple conserved domains, including Jumonji, ARID, and PHD zinc-finger motifs.
• Ubiquitously expressed with highest levels in testis; down-regulated in melanoma and glioblastoma but up-regulated in breast cancer.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1400–1500 of human JARID1B, a region without known functional domains; consider this when designing complementary detection reagents.
• For Western blot analysis, a specific band at approximately 176 kDa is expected; validate size and specificity in your sample system using appropriate controls.
• When performing ELISA, ensure that the coating antigen or capture antibody does not compete with the immunogen region to avoid signal interference.
• Reported cross-reactivity includes human, mouse, and rat; if using other species, verify reactivity experimentally.
• Due to the context-dependent expression in cancers, consider using relevant cancer cell lines or tissue panels for assay optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined procurement platform for IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare-target sourcing. This anti-JARID1B antibody supports epigenetic and oncology research applications, and we can assist with larger quantities or customized reagent needs. Contact us to simplify your supply chain from concept to clinic.