Anti-JAM2 Rabbit Polyclonal Antibody for WB and ELISA - P57087

Core Product Specifications and Parameters

Biological Background: JAM2 Function and Localization

• JAM2, also known as JAM-B, VE-JAM, or CD322, mediates heterotypic cell-cell interactions with its receptor JAM3 to regulate various cellular processes. Related references: PMID:11590146 PMID:11823489 PMID:24357068
• It contributes to hematopoietic stem and progenitor cell retention in the bone marrow via JAM3 interactions. Related references: PMID:11590146 PMID:24357068
• JAM2 is a key player in leukocyte extravasation, facilitating tethering, rolling, and transmigration; tethering/rolling requires integrin alpha-4/beta-1 binding. Related references: PMID:12239159 PMID:12070135
• It localizes at the cell membrane, cell junctions, and tight junctions.
• JAM2 is highly expressed in heart, placenta, lung, foreskin, lymph node, and caudate nuclei, and is prominently displayed on high endothelial venules. Related references: PMID:10779521 PMID:10945976 PMID:31851307
• The protein undergoes alternative splicing, is glycosylated, and contains a disulfide-bonded immunoglobulin domain.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular region (aa 29–238) of human JAM2, making it suitable for detecting both membrane-bound and soluble JAM2 forms.
• In Western blot, a predominant band at ~33 kDa is expected; be aware that post‑translational modifications (e.g., glycosylation) can alter electrophoretic mobility. Validate with appropriate molecular weight markers and controls.
• The antibody cross‑reacts with mouse and rat JAM2, offering potential for translational or multi‑species studies; nevertheless, verify reactivity in your experimental system.
• The reported ELISA application indicates the antibody can recognize native JAM2, supporting its use in immunoassays; consider pairing with a suitable detection format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your worldwide one‑stop partner for IVD raw‑material sourcing, covering everything from concept to clinic. This JAM2 polyclonal antibody is part of our extensive portfolio; we also supply validated antibody pairs, bulk reagents, and technical support to help you advance tight junction biology and leukocyte trafficking studies. Reach out to explore how we can tailor our offerings to your project needs.