Anti-JAM-A/CD321/F11R Rabbit PolymAb® Antibody for WB, IF/ICC, ELISA - Q9Y624 / O88792

Core Product Specifications and Parameters

Biological Background: JAM-A Function and Localization

• JAM-A (junctional adhesion molecule A), also called JAM-1 or F11 receptor, is an immunoglobulin superfamily protein that mediates homophilic cell adhesion and regulates tight junction assembly. It appears early in primordial cell junctions and recruits PARD3 to the site of cell-cell contact. Related references: PMID:11489913
• The protein acts as a ligand for integrin alpha-L/beta-2 (LFA-1), thereby facilitating memory T-cell and neutrophil transmigration across endothelial and epithelial barriers. Related references: PMID:11812992
• In platelets, JAM-A plays a role in platelet activation and aggregation, contributing to thrombus formation. Related references: PMID:10753840
• (Microbial infection) JAM-A serves as a cellular receptor for mammalian reovirus sigma-1 and for human rotavirus strain Wa, mediating viral entry into host cells.
• Subcellularly, JAM-A localizes to the cell membrane and is concentrated at tight junctions, consistent with its role in cell-cell adhesion.
• It is expressed in endothelial cells, epithelial cells, and leukocytes, highlighting its importance in vascular biology, barrier function, and immune cell trafficking.
• The protein undergoes post-translational modifications including phosphorylation, glycosylation, and disulfide bond formation, which may regulate its function and interactions.

Experimental Guidance and Technical Tips

• For Western blotting, a band at approximately 32 kDa is expected in human, mouse, and rat lysates. Consider running appropriate positive and negative controls, and validate expression in your target tissue or cell line.
• In immunofluorescence/ICC, JAM-A should exhibit membrane and junctional staining. Optimize fixation and permeabilization conditions, as tight junction proteins can be sensitive to over-fixation.
• For ELISA applications, the antibody can be used as a capture or detection reagent. If developing a sandwich ELISA, pair with a validated matched antibody to ensure specificity and sensitivity.
• The recombinant protein immunogen suggests the antibody may recognize both native and denatured epitopes, but empirical validation in each assay format is recommended.
• Reported cross-reactivity with rat is based on sequence homology; however, confirm performance in rat samples through pilot experiments if this is critical to your workflow.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a reliable, one-stop channel for IVD raw materials, including validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents. For targets like JAM-A—involved in tight junction biology, platelet function, and viral pathogenesis—we offer comprehensive sourcing support to help streamline your assay development from concept to clinic.