Anti-JAM-A/CD321/F11R Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y624

Core Product Specifications and Parameters

Biological Background: JAM-A Function and Localization

• JAM-A (F11R) is a single-pass type I membrane protein containing an immunoglobulin domain, essential for tight junction assembly and cell adhesion. It recruits PARD3 to primordial cell junctions, and its association with the PARD6-PARD3 complex may regulate tight junction formation (PubMed:11489913).
  Related references: PMID:11489913
• Through interaction with integrin alpha-L/beta-2, JAM-A regulates monocyte and neutrophil transmigration (PubMed:11812992), and it is involved in platelet activation (PubMed:10753840).
  Related references: PMID:11812992, PMID:10753840
• JAM-A localizes to tight junctions and the cell membrane.
• It undergoes post-translational modifications including disulfide bond formation, glycosylation, and phosphorylation.
• Expressed in endothelium, epithelium, and leukocytes (at protein level).
• JAM-A acts as a receptor for Mammalian reovirus sigma-1 and Human Rotavirus strain Wa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 28-238), so the antibody is likely to detect the extracellular region; confirm if membrane fractionation or permeabilization is needed for intracellular detection.
• For Western blot, a band around 33 kDa is expected; validate molecular weight against known positive controls and consider using reducing conditions.
• For IHC-P, optimize antigen retrieval and antibody dilution; tissue from human, mouse, or rat may be suitable.
• For ELISA, standard protocols may be applied; batch variability should be assessed.
• Since cross-reactivity with mouse and rat is reported, the antibody can be considered for cross-species studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you're developing assays for tight junction proteins or immune cell trafficking markers, CamelBio helps bridge the gap from concept to clinic. For JAM-A and other cell adhesion targets, we provide customized sourcing and technical support to accelerate your diagnostic development.