Anti-JAM-A/CD321/F11R Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q9Y624

Core Product Specifications and Parameters

Biological Background: JAM-A/CD321/F11R Function and Localization

• JAM-A, also known as F11 receptor or CD321, is a type I transmembrane glycoprotein member of the immunoglobulin superfamily, encoded by the F11R gene.
• In epithelial cells, JAM-A localizes to tight junctions where it nucleates junction assembly by recruiting the polarity protein PARD3, a process critical for maintaining paracellular barrier integrity (PubMed:11489913). Related references: PMID:11489913
• As a counter-receptor for leukocyte integrin LFA-1 (αLβ2), JAM-A facilitates directed migration of memory T cells and neutrophils across endothelia and epithelia during inflammation (PubMed:11812992). Related references: PMID:11812992
• On platelets, JAM-A (F11R) mediates platelet adhesion and activation through homophilic interactions and signaling (PubMed:10753840). Related references: PMID:10753840
• JAM-A also serves as a cellular receptor for pathogenic reovirus and rotavirus, mediating viral entry into host cells.
• Subcellularly, JAM-A is concentrated at tight junctions of the cell membrane in polarized epithelia and endothelia, and is expressed in tissues such as the vascular endothelium, epithelial linings, and circulating leukocytes.
• The protein undergoes N-glycosylation and phosphorylation, and its extracellular domain contains two Ig-like loops stabilized by disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant human full‑length JAM‑A protein; consider validating specificity by comparing staining in wild‑type vs knockout cell models.
• For Western blotting, a band at ~33 kDa is expected under reducing conditions; assess expression in relevant human cell lines such as HUVEC (endothelial) or Caco‑2 (epithelial).
• In immunofluorescence, a characteristic chicken‑wire pattern at intercellular borders indicates proper tight‑junction localization; optimize fixation and permeabilization for the target cell type.
• ELISA applications may benefit from pairing with a validated detection antibody; test serial dilutions of purified recombinant protein or cell lysates as positive controls.
• The antibody is specific for human JAM‑A and does not cross‑react with mouse or rat orthologues; confirm species compatibility before use in non‑human systems.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research laboratories, and biotech firms a streamlined procurement channel for high‑quality IVD raw materials like this anti‑JAM‑A rabbit monoclonal antibody. We supply validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare or difficult‑to‑obtain targets, supporting every stage from concept to clinic. Whether you need reproducible immunoassay components, technical consultation, or a partner to accelerate your IVD development pipeline, CamelBio is your single‑point resource for all raw‑material requirements.