Anti-JAM-A/CD321/F11R Monoclonal Antibody for FC - Q9Y624

Core Product Specifications and Parameters

Biological Background: JAM-A Function and Localization

• JAM-A (F11R/JAM1) is a single-pass type I membrane protein belonging to the immunoglobulin superfamily; alternative splicing generates multiple isoforms.
• Plays a critical role in epithelial tight junction assembly by recruiting the polarity protein PARD3 early in junction formation (PubMed:11489913). Related references: PMID:11489913
• Functions as a ligand for integrin alpha-L/beta-2 (LFA-1), mediating memory T-cell and neutrophil transmigration across endothelial and epithelial barriers (PubMed:11812992). Related references: PMID:11812992
• Involved in platelet activation and aggregation (PubMed:10753840). Related references: PMID:10753840
• Acts as a receptor for Mammalian reovirus sigma-1 and Human Rotavirus strain Wa, facilitating viral entry.
• Subcellularly localizes to tight junctions and the cell membrane, with expression observed in endothelium, epithelium, and leukocytes.
• Post-translational modifications include N-glycosylation, phosphorylation, and conserved disulfide bonds within its extracellular Ig domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 28-242), suggesting the antibody likely recognizes an extracellular epitope; therefore, it may be suitable for live-cell flow cytometry without permeabilization.
• Titrate the antibody to determine the optimal concentration for your specific cell type, and always include appropriate isotype controls.
• As JAM-A is expressed on endothelial and epithelial cells as well as leukocytes, validate performance in the relevant sample system.
• Avoid harsh fixation or permeabilization that might mask the epitope; if using fixed cells, test antigen retrieval methods if signal is low.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to validated IVD raw materials, including this high-quality monoclonal antibody for JAM-A research. Beyond antibodies, our supply capabilities cover optimized antibody pairs, bulk ancillary reagents, and assisted sourcing of rare targets, supporting your work on tight junction biology, immunology, and viral pathogenesis. Let us bridge the gap from concept to clinic with reliable raw-material solutions.