Anti-JAM-A/CD321 Rabbit Polyclonal Antibody for WB, ELISA - Q92993

Core Product Specifications and Parameters

Biological Background: JAM-A/CD321 Function and Localization

• Catalytic subunit of the NuA4 histone acetyltransferase complex; acetylates histones H2A and H4 to regulate transcriptional activation, DNA repair, and apoptosis. Related references: PMID:12776177, PMID:14966270, PMID:15042092
• Directly facilitates DNA double-strand break repair via homologous recombination; catalyzes H2AK15 acetylation to block TP53BP1 chromatin association and H2AXK5 acetylation to promote NBN assembly. Related references: PMID:27153538, PMID:32832608, PMID:17709392, PMID:26438602
• Acetylates a broad spectrum of non-histone substrates; for instance, modifies ATM to activate the DNA damage response and FOXP3 to enhance regulatory T-cell function. Related references: PMID:16141325, PMID:17360565
• Plays a key mitotic role by acetylating AURKB, RAN, and NDC80 to ensure accurate chromosome segregation and robust kinetochore–microtubule attachments. Related references: PMID:26829474, PMID:29040603, PMID:30409912
• Localizes to various cellular compartments including the nucleus, chromosomes, kinetochores, and cytoplasm; enriched at DNA damage sites and mitotic structures.
• Its acetyltransferase activity is regulated by phosphorylation; GSK3-mediated phosphorylation at Ser-86 triggers autophagy-related acetylation of ULK1. Related references: PMID:30704899
• Exhibits acyltransferase versatility, utilizing crotonyl-CoA, lactyl-CoA, and 2-hydroxyisobutanoyl-CoA to catalyze protein crotonylation, lactylation, and 2-hydroxyisobutyrylation, respectively. Related references: PMID:29192674, PMID:34608293, PMID:38961290

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain of mouse JAM-A (aa 27–242); the polyclonal nature suggests recognition of multiple epitopes.
• For Western blot, a predominant band around 32 kDa is expected; validate with positive control lysates from mouse, human, or rat tissues known to express JAM-A.
• In ELISA applications, use the recombinant immunogen or a closely related fusion protein as a coating standard to ensure specificity.
• Although validated for WB and ELISA, the antibody may be compatible with other techniques (e.g., IHC, IF) under optimized conditions; consider antigen retrieval and appropriate blocking steps.
• Since cross-reactivity is reported across human, mouse, and rat, confirm species-specific reactivity in your assay system using appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. The JAM‑A/CD321 antibody supports research in cell adhesion, tight junction biology, and inflammation. Beyond this product, our supply capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing, all designed to streamline your assay development workflows.