Anti-JAK3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P52333

Core Product Specifications and Parameters

Biological Background: JAK3 Function and Localization

• JAK3 (Janus kinase 3) is a non-receptor tyrosine-protein kinase encoded by the JAK3 gene in humans. Alternative splicing produces two isoforms: the longer S-form and the shorter B-form, which differ in tissue distribution.
• It plays a central role in cytokine signal transduction by associating with type I receptors that share the common gamma chain (IL2RG), including receptors for IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21.
• Upon ligand binding, JAK3 phosphorylates tyrosine residues on the receptor cytoplasmic tail, creating docking sites for STAT transcription factors; it then phosphorylates STATs (e.g., STAT5A/B), which dimerize and translocate to the nucleus to regulate gene expression.
• JAK3 is essential for both innate and adaptive immunity, and is critically involved in T-cell development and hematopoiesis.
• Subcellularly, JAK3 is localized to the cytoplasm and is associated with the endomembrane system.
• Tissue expression: JAK3 is detected in NK cells and NK-like cell lines, but not in resting T-cells. The S-form predominates in hematopoietic lineages, while the B-form is found in both hematopoietic and epithelial cells.
• The protein belongs to the protein kinase superfamily, Tyr protein kinase family, and JAK subfamily. It contains an SH2 domain and a tyrosine-protein kinase domain.
• Post-translational modifications include phosphorylation (it is a phosphoprotein). Keywords also highlight its involvement in disease variants and severe combined immunodeficiency (SCID).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 350-530 of human JAK3. For Western blot, expect a band at approximately 125 kDa.
• For immunofluorescence/immunocytochemistry, consider fixing and permeabilization protocols suitable for detection of cytoplasmic proteins.
• When performing ELISA, use appropriate blocking and detection systems; pairing with another JAK3 antibody may be beneficial for sandwich assays.
• As a polyclonal antibody, lot-to-lot variability may affect performance; validation in the relevant sample system is recommended.
• Note that JAK3 is primarily expressed in immune cells; positive controls could include NK cell lines or activated T-cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop IVD raw-material partner offering a comprehensive portfolio of antibodies, antigens, and ancillary reagents. In addition to this JAK3 polyclonal antibody, we supply validated antibody pairs, expression-optimized monoclonal antibodies, and bulk quantities of critical raw materials for immunology and inflammation research. Our sourcing network also supports customization and rapid access to difficult-to-find targets, helping diagnostic developers accelerate from concept to clinic.