Anti-JAK1 Polyclonal Antibody for WB, IF/ICC, ELISA - P23458

Core Product Specifications and Parameters

Biological Background: JAK1 Function and Localization

• JAK1 is a non-membrane spanning protein tyrosine kinase that phosphorylates signal-transducing subunits of cytokine receptor complexes, including IL2RB, IL10RA, IFNAR2, IL6ST, LIFR, OSMR, and IL31RA. Related references: PMID:11909529, PMID:12133952, PMID:15194700
• It is a key mediator in the IFN-alpha/beta/gamma signaling pathway. Upon interferon binding to the IFNAR1-IFNAR2 heterodimer, JAK1 phosphorylates IFNAR2, creating docking sites for STAT proteins and directly phosphorylating STATs. Related references: PMID:7759950, PMID:16239216, PMID:28111307, PMID:32750333, PMID:8232552
• JAK1 is involved in MT-RNR2/humanin-mediated signaling, leading to STAT3 phosphorylation. Related references: PMID:27384491
• Binding of CNTF or the CLCF1/CLF heterodimer to CNTFR leads to IL6ST/gp130-LIFR dimerization followed by activation of JAK1 and JAK2, which in turn phosphorylate IL6ST/gp130 and LIFR, recruiting STAT3. Related references: PMID:11294841
• JAK1 participates in oncostatin-M signaling through both type I (LIFR/IL6ST) and type II (OSMR/IL6ST) receptor complexes. Related references: PMID:9188471
• It mediates interleukin-31 signaling via the IL31 receptor complex (OSMR/IL31RA). Related references: PMID:15194700
• JAK1 is localized to the endomembrane system and is expressed at higher levels in primary colon tumors than in normal colon tissue.
• Additional keywords associated with JAK1 include acetylation, ATP-binding, kinase, magnesium, membrane, metal-binding, nucleotide-binding, phosphoprotein, proteomics identification, reference proteome, repeat, SH2 domain, transferase, tyrosine-protein kinase, and Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 30–300; the antibody may detect denatured JAK1 in WB under reducing conditions, but validation is recommended for each application.
• For IF/ICC, test different fixation and permeabilization protocols to optimize epitope accessibility, as the epitope may be masked in native conformations.
• For ELISA, consider pairing with a detection antibody that binds a distinct epitope; validate in the relevant sample matrix to ensure specificity.
• Confirm reactivity with endogenous JAK1 in human, mouse, and rat cell lysates; cross-reactivity may vary depending on sample preparation and detection system.
• The predicted molecular weight is 133 kDa; ensure efficient transfer for high-molecular-weight proteins in WB and consider using appropriate positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with validated IVD raw materials, including optimized monoclonal and polyclonal antibodies, antibody pairs, and ancillary reagents for cytokine and kinase pathway assay development. For this anti-JAK1 polyclonal antibody, we support your transition from concept to clinic with bulk sourcing, custom formulations, and access to rare-target raw materials. Contact our specialists to discuss your project needs and supply requirements.