Anti-JAG2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y219

Core Product Specifications and Parameters

Biological Background: JAG2 Function and Localization

• JAG2 encodes Protein jagged-2, a single-pass type I transmembrane protein that belongs to the Jagged family of Notch ligands.
• It contains a signal peptide, multiple EGF-like repeats, a cysteine-rich domain, and a cytoplasmic tail, with predicted molecular weight of approximately 133 kDa.
• As a putative Notch ligand, JAG2 mediates cell–cell communication through the Notch signaling pathway, influencing cell fate decisions, proliferation, and differentiation.
• JAG2 is involved in limb development and likely plays broader roles in embryonic patterning and tissue morphogenesis.
• Subcellularly, JAG2 localizes to the plasma membrane, consistent with its role in receptor–ligand interactions at the cell surface.
• Tissue expression is predominant in heart, placenta, and skeletal muscle, lower in pancreas, and very low in brain, lung, liver, and kidney.
• Mutations in JAG2 are associated with limb-girdle muscular dystrophy (LGMDR27), highlighting its importance in muscle maintenance.
• Alternative splicing produces multiple isoforms, and the protein undergoes glycosylation and phosphorylation, which may modulate its activity.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal extracellular region (amino acids 24–200), potentially recognizing the mature ligand exposed on the cell surface.
• For Western blot, a band around 133 kDa is expected; validate with positive lysates from JAG2-expressing tissues such as heart or skeletal muscle.
• In immunofluorescence/ICC, expect membrane staining; include appropriate permeabilization steps to detect the intracellular epitope if required.
• Cross-reactivity with mouse and rat enables comparative studies; however, always confirm specificity in the respective species.
• ELISA applications can be explored; consider pairing this antibody with a detection antibody targeting a distinct epitope for sandwich assays.
• Alternative splicing and post-translational modifications may result in multiple bands; use freshly prepared lysates and protease/phosphatase inhibitors.
• Store aliquots upon arrival at -20°C or -80°C as recommended; avoid repeated freeze–thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-JAG2 antibody supports developmental biology and muscular dystrophy research, and we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your workflows. Partner with CamelBio to accelerate assay development and secure a reliable supply chain.