Anti-JADE1 Rabbit Polyclonal Antibody for IF/ICC, ELISA - Q6IE81

Core Product Specifications and Parameters

Biological Background: JADE1 Function and Localization

• Protein Jade-1 (JADE1, also known as PHF17) is a PHD finger protein and scaffold subunit of HBO1 histone acetyltransferase complexes, featuring zinc-finger domains for chromatin association. Related references: PMID:16387653 PMID:19187766 PMID:20129055
• Directs KAT7/HBO1 to acetylate histone H4 at K5, K8, and K12, controlling DNA replication initiation. Related references: PMID:20129055 PMID:24065767
• May also support histone H4 acetylation by KAT5, potentially influencing transcription. Related references: PMID:15502158
• Promotes apoptosis and is considered a renal tumor suppressor. Related references: PMID:16046545
• Inhibits canonical Wnt signaling through interactions including NPHP4. Related references: PMID:22654112
• Resides in the nucleus, chromosomes, cytoplasm, cytoskeleton, and cilium basal body.
• Predominantly expressed in kidney, with lower levels in pancreas, liver, and heart; downregulated in renal cancers.
• Subject to phosphorylation, acetylation, and ubiquitination, as indicated by proteomics and functional studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 210–509 of human JADE1 (NP_079176.2), covering the central region; this design may detect multiple isoforms or cleavage products.
• For immunofluorescence (IF/ICC), optimize cell permeabilization to visualize both nuclear and cytoplasmic pools; appropriate controls are recommended.
• ELISA performance should be validated with recombinant JADE1 protein or cell lysates from human/mouse samples to confirm sensitivity and specificity.
• Consider testing antibody reactivity in kidney-derived cell lines, where JADE1 expression is highest, and in renal cancer models with reduced expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for targets like JADE1. As a streamlined sourcing partner, we can also assist in procuring rare-target raw materials and supplying technical support from concept to clinic. Our commitment is to deliver reliable, cost-effective solutions that accelerate your assay development for chromatin biology and cancer research applications.