Anti-JAB1/CSN5/COPS5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q92905

Core Product Specifications and Parameters

Biological Background: JAB1/CSN5/COPS5 Function and Localization

• COP9 signalosome complex subunit 5 (CSN5), also known as Jun activation domain-binding protein 1 (JAB1), is encoded by the COPS5 gene in humans.
• As a subunit of the COP9 signalosome (CSN) complex, CSN5 plays a critical role in regulating the ubiquitin-proteasome system by mediating the deneddylation of cullin subunits of SCF-type E3 ligase complexes, thereby decreasing their ubiquitin ligase activity.
• CSN5 likely acts as the catalytic center of the CSN complex, responsible for cleaving the ubiquitin-like protein NEDD8 from cullins; however, it requires other CSN subunits for full protease activity.
• The CSN complex, via CSN5, is also involved in the phosphorylation of key regulatory proteins, including p53/TP53, c-Jun/JUN, IκBα/NFKBIA, ITPK1, and IRF8, potentially through associated kinases such as CK2 and PKD.
• CSN5 directly interacts with numerous proteins regulated by the CSN complex, including promoting the proteasomal degradation of BRSK2, underscoring its central role in protein stability control.
• Subcellularly, CSN5 localizes to the cytoplasm, cytosol, nucleus, perinuclear region, and cytoplasmic vesicles including secretory and synaptic vesicles.
• Key structural and functional features of CSN5 include its metalloprotease activity dependent on zinc binding, and post-translational acetylation.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was generated against a recombinant protein corresponding to amino acids 1-334 of human CSN5, covering the full-length protein, which may recognize multiple epitopes; consider validating specificity in your target species using knockout or knockdown controls.
• Recommended applications include Western blotting (WB) detecting a band at ~38 kDa, immunofluorescence/immunocytochemistry (IF/ICC), and ELISA; optimize dilutions and conditions for your specific experimental setup.
• Due to high sequence similarity, this antibody cross-reacts with mouse and rat CSN5; confirm expected molecular weight and localization in these species.
• For WB, use appropriate loading controls and consider that CSN5 expression may vary across tissues and cell lines; nuclear and cytoplasmic extracts can be tested to confirm subcellular distribution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing for proteins like CSN5. As a one-stop partner spanning concept to clinic, we support your development of assays targeting ubiquitin-proteasome pathway components with reliable high-quality materials.