Anti-JAB1/CSN5/COPS5 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA, ChIP - Q92905

Core Product Specifications and Parameters

Biological Background: JAB1/CSN5/COPS5 Function and Localization

• JAB1/CSN5/COPS5, also known as COP9 signalosome complex subunit 5 or Jun activation domain-binding protein 1, is a core component of the COP9 signalosome (CSN) complex.
• The CSN complex is an essential regulator of the ubiquitin-proteasome system, mediating deneddylation of cullin subunits of SCF-type E3 ligases, thereby modulating ubiquitin ligase activity.
• As the probable catalytic subunit, JAB1/CSN5/COPS5 is a metalloprotease that cleaves Nedd8 from cullins, requiring other CSN subunits for activity; it also interacts directly with numerous CSN-regulated proteins.
• The protein is involved in phosphorylation of key signaling molecules (p53, c-Jun, IκBα, ITPK1, IRF8), promoting their ubiquitin-dependent degradation, and facilitating proteasomal degradation of BRSK2.
• Subcellular localization includes cytoplasm, cytosol, nucleus, perinuclear region, and cytoplasmic vesicles such as secretory and synaptic vesicles, reflecting its diverse functional roles.
• Keywords include hydrolase, metalloprotease, signalosome, and zinc-binding, with post-translational modifications such as acetylation noted.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-100 of human JAB1/CSN5/COPS5, targeting the N-terminal region; consider that this region may be conserved across species, consistent with reported cross-reactivity.
• Rabbit monoclonal antibodies typically offer high specificity and lot-to-lot consistency; for applications like ChIP, ensure proper antibody validation in your specific chromatin preparation conditions.
• Given the protein's presence in both cytoplasm and nucleus, subcellular fractionation or colocalization studies (IF/ICC) may require careful optimization of permeabilization and fixation protocols.
• Recommended to perform a pilot dilution series for each application; for Western blot, the expected band is ~38 kDa, but post-translational modifications may cause minor shifts.

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