Anti-IVD Polyclonal Antibody for WB, IHC-P, ELISA - P26440

Core Product Specifications and Parameters

Biological Background: Isovaleryl-CoA Dehydrogenase (IVD) Function and Localization

• IVD encodes isovaleryl-CoA dehydrogenase, a mitochondrial matrix enzyme also known as butyryl-CoA dehydrogenase.
• It catalyzes the third step in leucine catabolism, converting isovaleryl-CoA to 3-methylbut-2-enoyl-CoA, and also participates in the beta-oxidation of short-chain fatty acids via dehydrogenation of saturated short-chain acyl-CoA thioesters (e.g., pentanoyl-CoA, hexanoyl-CoA, butanoyl-CoA) using electron transfer flavoprotein (ETF) as an electron acceptor.
  Related references: PMID:7640268
• The protein belongs to the acyl-CoA dehydrogenase family and is an FAD-dependent oxidoreductase.
• Immunofluorescence and biochemical studies localize IVD predominantly to the mitochondrial matrix.
• UniProt annotations list key post-translational modifications such as acetylation, along with potential alternative splicing.
• Disease-associated mutations in IVD are linked to isovaleric acidemia, a rare metabolic disorder.

Experimental Guidance and Technical Tips

• The immunogen, spanning amino acids 33–200 of human IVD, likely targets a conserved N-terminal domain, consistent with observed cross-reactivity to mouse and rat.
• For Western blotting, expect a band near 47 kDa (mature mitochondrial form ~46.7 kDa); use positive controls such as liver or kidney lysates and validate in your specific sample system.
• IHC-P may show mitochondrial staining in metabolically active tissues; confirm specificity via blocking peptide or knockout/knockdown models if available.
• The polyclonal format can serve as capture or detection antibody in sandwich ELISA; test empirically for optimal orientation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs integrated access to IVD raw materials, from antibody generation to bulk reagent supply. For isovaleryl-CoA dehydrogenase targets and metabolic disease research, our platform provides validated polyclonal antibodies, antibody pairs, and ancillary reagents, complemented by technical consulting to support your development pipeline. Whether you need routine sourcing or rare target solutions, CamelBio bridges the gap from concept to clinic.