Anti-ITSN2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NZM3

Core Product Specifications and Parameters

Biological Background: Intersectin-2 Function and Localization

• ITSN2 (intersectin-2) is a multidomain adapter protein also known as SH3P18 or SWAP, with a molecular weight of approximately 193 kDa.
• It contains SH3 domains, a coiled-coil region, and calcium-binding motifs, and belongs to the intersectin family of endocytic scaffold proteins.
• ITSN2 provides an indirect link between endocytic membrane traffic and the actin assembly machinery, regulating the formation and maturation of clathrin-coated vesicles (CCVs).
• Plays a role in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR), facilitating cargo internalization.
• Involved in dendrite formation by melanocytes, as evidenced by functional studies. Related references: PMID:23999003
• Localizes to the cytoplasm, consistent with its role in endocytic trafficking.
• Expressed ubiquitously; isoform 1 is predominantly expressed in adult heart and liver, and it is also expressed in melanocytes.
• Post-translational modifications include phosphorylation, and the gene undergoes alternative splicing to generate multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 500–620 of human ITSN2; consider verifying specificity against closely related intersectin-1 or other SH3 domain proteins if cross-reactivity is a concern.
• For Western blot (WB), a specific band around 193 kDa is expected; optimize sample preparation and antibody dilution to achieve clear detection.
• In immunofluorescence (IF/ICC), a cytoplasmic staining pattern should be observed, consistent with ITSN2 localization.
• The antibody is applicable to ELISA; validate the assay format and sensitivity according to your experimental requirements.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a one-stop sourcing platform for IVD raw materials, including validated antibodies for endocytosis and actin dynamics targets such as ITSN2. Our supply chain covers optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets, supporting your end-to-end assay development from concept to clinic.