Anti-ITPRIP Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8IWB1

Core Product Specifications and Parameters

Biological Background: ITPRIP Function and Localization

• ITPRIP (also known as DANGER) is an inositol 1,4,5-trisphosphate receptor-interacting protein encoded by the ITPRIP gene in humans.
• It enhances Ca²⁺-mediated inhibition of ITPR Ca²⁺ release, modulating intracellular calcium signaling.
• The protein localizes to the cell membrane and the nucleus outer membrane, suggesting dual roles in membrane-associated and nuclear processes.
• Predicted structural features include a signal peptide, a coiled-coil domain, and a transmembrane helix, consistent with its membrane localization.
• ITPRIP is a glycoprotein and phosphoprotein, as identified by proteomics, indicating post-translational modifications that may regulate its function.
• Tissue expression is detected in the brain, with pronounced concentration in cerebellar Purkinje cells at the protein level.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment (amino acids 101–290) of human ITPRIP, likely spanning an extracellular or cytoplasmic region outside the predicted transmembrane domain, which may favor native epitope recognition in IF/ICC assays.
• As a polyclonal antibody, it may recognize multiple epitopes; consider potential cross-reactivity with other ITPRIP isoforms or family members if present in your sample.
• For WB, the predicted molecular weight is approximately 62 kDa; validate bands in appropriate lysates (e.g., human brain tissue or ITPRIP-expressing cell lines).
• Optimize fixation and permeabilization conditions for IF/ICC to preserve both membrane and nuclear localization signals.
• This antibody is suitable for ELISA; pairing with monoclonal antibodies may support the development of a sandwich ELISA.

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