Anti-ITPR3 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q14573

Core Product Specifications and Parameters

Biological Background: ITPR3 Function and Localization

• ITPR3 encodes inositol 1,4,5-trisphosphate receptor type 3 (IP3R-3), a member of the inositol 1,4,5-trisphosphate receptor (IP3R) family of intracellular calcium release channels.
• The ITPR3 protein functions as an IP3-gated calcium channel, releasing Ca²⁺ from the endoplasmic reticulum into the cytoplasm upon IP3 binding, thereby regulating cellular calcium homeostasis.
  Related references: PMID:8081734, PMID:32949214, PMID:37898605
• Structural studies reveal a gating mechanism where IP3 binding stabilizes a preactivated state, enhancing calcium sensitivity; calcium binding at the juxtamembrane domain then promotes channel opening, while high cytosolic calcium later binds to a cytoplasmic domain to inhibit the channel.
  Related references: PMID:30013099, PMID:35301323, PMID:37898605
• ITPR3 localizes primarily to the endoplasmic reticulum membrane and is also found in cytoplasmic vesicles and secretory vesicle membranes.
• Tissue expression of ITPR3 is prominent in intestinal crypt and villus epithelial cells.
• Mutations in ITPR3 are associated with Charcot-Marie-Tooth disease and ectodermal dysplasia, highlighting its clinical significance.
• The protein is a phosphoprotein with a large molecular weight of approximately 304 kDa.

Experimental Guidance and Technical Tips

• Given the large protein weight (304 kDa), consider using a low-percentage SDS-PAGE gel (e.g., 6%) and extended transfer times for Western blotting.
• For immunofluorescence (IF/ICC), permeabilization with detergents such as Triton X-100 or digitonin may be required to allow antibody access to the endoplasmic reticulum.
• The immunogen spanning amino acids 1630–1870 targets the C-terminal region of ITPR3, which may be useful for detecting full-length protein or specific fragments.
• Validate the antibody in your specific sample system; optimal working dilutions for each application should be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. With a focus on calcium signaling research, we offer comprehensive support to streamline your transition from concept to clinic. Contact us to secure high-quality ITPR3 antibodies and related reagents for your diagnostic development needs.