Anti-ITPR2 Polyclonal Antibody for IHC-P, IF/ICC, ELISA - Q14571

Core Product Specifications and Parameters

Biological Background: Inositol 1,4,5-trisphosphate-gated Calcium Channel ITPR2 Function and Localization

• ITPR2 encodes inositol 1,4,5-trisphosphate receptor type 2 (IP3R2), a ligand-gated calcium channel that releases Ca²⁺ from the endoplasmic reticulum lumen into the cytoplasm upon binding of IP3.
• The protein exists in two functional states: a long-lived closed "parked" state and a bursting "drive" mode, with ligands facilitating the transition between these states (By similarity).
• ITPR2 belongs to the IP3 receptor family and contains ATP-binding, calcium-binding, and zinc-binding sites, as well as multiple transmembrane helices.
• Subcellularly, ITPR2 is primarily located in the endoplasmic reticulum membrane and is also detected in cytoplasmic vesicles and secretory vesicle membranes.
• Tissue expression is enriched in skeletal muscle and heart, where it plays a critical role in excitation-contraction coupling and calcium signaling.
• The protein is subject to alternative splicing and phosphorylation, with proteomics identification of phosphosites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 2415-2480) of ITPR2, which is expected to be exposed to the cytoplasm; this polyclonal antibody may recognize both full-length and truncated isoforms.
• For IHC-P on paraffin-embedded tissues, consider using antigen retrieval and validate in known positive tissues such as skeletal muscle or heart.
• For IF/ICC, optimize fixation and permeabilization to ensure accessibility to the endoplasmic reticulum membrane-localized target.
• In ELISA, ensure proper coating of the recombinant protein or cell lysates to capture the antibody; cross-reactivity with mouse and rat suggests suitability for multi-species studies.
• As a polyclonal antibody, lot-to-lot consistency should be verified, and appropriate controls (e.g., peptide blocking) are recommended for critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-ITPR2 polyclonal antibody supports research into calcium signaling and muscle physiology. Alongside validated antibodies, we supply optimized antibody pairs, bulk ancillary reagents, and can source rare-target raw materials to accelerate your diagnostic development.