Anti-ITPKB Rabbit Polyclonal Antibody for WB and ELISA - P27987

Core Product Specifications and Parameters

Biological Background: ITPKB Function and Localization

• ITPKB (Inositol-trisphosphate 3-kinase B) is also known as IP3K-B, IP3KB, PIG37, and IP3-3KB. It is a member of the inositol phosphate kinase family that converts inositol 1,4,5-trisphosphate (InsP3) into inositol 1,3,4,5-tetrakisphosphate (InsP4).
• The enzyme participates in the regulation of calcium homeostasis by generating InsP4, which may act as a second messenger modulating intracellular calcium levels.
• ITPKB localizes primarily to the cytoplasm and is associated with the cytoskeleton and endoplasmic reticulum, indicating roles in both cytoskeletal organization and calcium store regulation.
• UniProt keywords indicate ITPKB is an ATP-binding, calmodulin-binding kinase and transferase, and is subject to alternative splicing and phosphorylation, highlighting regulatory control.
• The protein has a molecular weight of approximately 102 kDa, consistent with the gene product of human ITPKB (Gene ID: 3707).
• The antibody's cross-reactivity with mouse and rat ITPKB reflects the high sequence homology of the N-terminal region (amino acids 1-260) used as immunogen, facilitating cross-species applications.

Experimental Guidance and Technical Tips

• For Western blot, a band of approximately 102 kDa is expected. Consider using reducing conditions and standard protein loading.
• For ELISA, the antibody can be used as capture or detection after appropriate titer determination; validate in relevant sample systems.
• Given the cytoplasmic and cytoskeletal localization, consider fractionating samples to enrich for target protein.
• The immunogen spans the N-terminal region, so the antibody may recognize multiple isoforms if alternative splicing occurs; verify specificity with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with one-stop access to rigorously validated IVD raw materials, including this anti-ITPKB polyclonal antibody for calcium signaling and inositol phosphate research. Our portfolio offers optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, supporting every stage from assay development to clinical translation.