Anti-ITPKA Rabbit Polyclonal Antibody for WB, ELISA - P23677

Core Product Specifications and Parameters

Biological Background: ITPKA Function and Localization

• ITPKA (inositol-trisphosphate 3-kinase A) is a kinase that phosphorylates 1D-myo-inositol 1,4,5-trisphosphate (InsP3) to produce 1D-myo-inositol 1,3,4,5-tetrakisphosphate, thereby modulating intracellular calcium signaling.
• The enzyme is a key regulator of calcium homeostasis, linking InsP3 metabolism to downstream calcium-dependent processes.
• ITPKA is predominantly expressed in the brain, suggesting a specialized role in neuronal calcium signaling.
• Subcellularly, ITPKA localizes to the cytoplasm and associates with the cytoskeleton.
• The protein contains ATP-binding and calmodulin-binding domains, and undergoes post-translational modifications such as methylation and phosphorylation.
• Alternative names for ITPKA include IP3KA and IP3-3KA, reflecting its enzymatic activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 141-461 of human ITPKA, covering the catalytic kinase domain; consider this region’s conservation when assessing cross-reactivity.
• For Western blot, a band at ~51 kDa is expected; validate in relevant tissue or cell lysates, especially brain-derived samples.
• When using this antibody in ELISA, include appropriate positive and negative controls to confirm specificity.
• Cross-reactivity with mouse and rat ITPKA is reported, but it is advisable to verify performance in the specific experimental system.
• Optimize antibody dilutions empirically for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers focusing on inositol phosphate signaling and calcium regulation, CamelBio supplies validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our comprehensive supply chain streamlines your development of reliable immunoassays and research tools.