Anti-ITK/EMT Monoclonal Antibody for WB, ELISA - Q08881

Core Product Specifications and Parameters

Biological Background: ITK/EMT Function and Localization

• Tyrosine-protein kinase ITK/TSK, also known as interleukin-2-inducible T-cell kinase, kinase EMT, T-cell-specific kinase, or tyrosine-protein kinase Lyk, is encoded by the ITK gene.
• Plays an essential role in adaptive immunity by regulating the development, function, and differentiation of conventional T-cells and nonconventional NKT-cells. Upon TCR engagement, ITK is recruited to the membrane, phosphorylated by LCK, and autophosphorylates to become fully active.
• Once activated, ITK phosphorylates PLCG1, leading to calcium release and NFAT nuclear translocation, and phosphorylates adapter proteins LAT and LCP2, ultimately driving lymphokine production, T-cell proliferation, and differentiation. Related references: PMID:12186560, PMID:12682224, PMID:21725281
• Localizes to both the cytoplasm and nucleus.
• Expressed in T-cell lines and natural killer cell lines.
• Phosphorylates TBX21 at Tyr-530, mediating its interaction with GATA3; also involved in gamma-delta T-cell calcium response and transcriptomic modulation.
• Contains SH2, SH3, and kinase domains with a zinc-finger motif; classified as a tyrosine-protein kinase with ATP-binding and metal-binding properties.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole cell lysates from human T-cell lines (e.g., Jurkat) to detect the ~72 kDa band.
• For ELISA, validate with recombinant ITK protein or cell lysates to assess binding specificity; include appropriate positive and negative controls.
• Given the immunogen is full-length recombinant protein, the antibody may recognize multiple epitopes; confirm specificity using knockout or knockdown models.
• Ensure sample preparation includes protease and phosphatase inhibitors to preserve phosphorylation states and protein integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw material sourcing, offering comprehensive support from target selection to clinical validation. This anti-ITK/EMT monoclonal antibody addresses research in T-cell signaling, adaptive immunity, and kinase inhibitor development. Beyond individual antibodies, we supply validated antibody pairs, optimized bulk reagents, and custom sourcing for rare targets to accelerate your diagnostic assay development.