Anti-ITIH3 Rabbit Polyclonal Antibody for WB, ELISA - Q06033

Core Product Specifications and Parameters

Biological Background: ITIH3 Function and Localization

• ITIH3 (Inter-alpha-trypsin inhibitor heavy chain H3) is also known as serum-derived hyaluronan-associated protein (SHAP) and is encoded by the ITIH3 gene. It belongs to the inter-alpha-trypsin inhibitor (ITI) family.
• ITIH3 may function as a carrier of hyaluronan (HA) in serum or as a binding protein that links HA to other matrix proteins, including cell surface proteins, thereby regulating HA localization, synthesis, and degradation—processes critical for cell migration and tissue remodeling.
• The protein is secreted and contains heavy chain domains that enable covalent linkage to hyaluronan via a serine ester bond, forming covalent complexes with HA.
• ITIH3 is classified as a serine protease inhibitor (serpin) family member, though its primary role is in extracellular matrix organization rather than protease inhibition; it features a signal peptide and multiple glycosylation sites.
• Alternative splicing of ITIH3 produces multiple isoforms, which may have distinct tissue distribution and functional properties.
• Key post-translational modifications include N-linked glycosylation, which influences protein stability and function.
• The protein has a predicted molecular weight of ~99.8 kDa, consistent with the observed ~100 kDa band in western blotting; it is expressed in liver and secreted into plasma, with broad tissue distribution due to its role in hyaluronan metabolism.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 35-180 of human ITIH3 (NP_002208.3), a region likely conserved across species, supporting the reported cross-reactivity with mouse and rat.
• For western blot (WB), consider a starting dilution range of 1:500–1:2000, though optimal dilution should be empirically determined in the target sample system. A band near 100 kDa is expected; additional bands may reflect isoforms or complexes.
• For ELISA, the antibody can be used as a detection or capture reagent; titrate to establish working concentrations for direct or sandwich formats.
• As ITIH3 is a secreted protein, serum, plasma, or cell culture supernatant may be suitable sample types. Pre-clearing or enrichment steps may improve detection.
• Validate the antibody’s performance in the specific application and species of interest with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including this ITIH3 rabbit polyclonal antibody, to support research in hyaluronan metabolism and extracellular matrix biology. Our portfolio spans validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents, all customizable to meet diagnostic manufacturers' and research laboratories' needs. We specialize in rare target raw-material sourcing and provide end-to-end support from concept to clinic.