Anti-ITGBL1 Rabbit Polyclonal Antibody for ELISA and WB - O95965

Core Product Specifications and Parameters

Biological Background: Integrin beta-like protein 1 (ITGBL1) Function and Localization

• ITGBL1, also known as Osteoblast-specific cysteine-rich protein, is encoded by the ITGBL1 gene. It is structurally related to integrin beta subunits but functions as a secreted factor rather than a transmembrane receptor.
• The protein contains multiple EGF-like repeat domains, which are commonly involved in protein-protein interactions and cell adhesion processes.
• ITGBL1 is widely expressed at low levels in many tissues, with readily detectable expression only in the aorta, suggesting a specific role in vascular biology.
• According to UniProt annotation, ITGBL1 undergoes alternative splicing, producing multiple isoforms that may have distinct functions.
• As a secreted glycoprotein with disulfide bonds, ITGBL1 is localized in the extracellular space, consistent with potential roles in extracellular matrix remodeling and intercellular signaling.
• Post-translational modifications, including glycosylation and disulfide bond formation, are critical for its structural stability and function.

Experimental Guidance and Technical Tips

• For Western blotting, denaturing and reducing conditions are recommended since the antibody was raised against a linear peptide epitope within amino acids 200-300.
• The predicted molecular weight is 54 kDa, but the observed band may appear higher due to glycosylation; verify in the relevant sample system.
• When performing ELISA, consider optimizing coating conditions (e.g., pH and buffer composition) to enhance peptide recognition.
• Cross-reactivity with mouse and rat ITGBL1 has been demonstrated, but species-specific sequence alignment of the immunogen region is advised to confirm epitope conservation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw material supplier, offering validated antibodies like this anti-ITGBL1 polyclonal antibody for research on integrin-related signaling and vascular biology. We provide one-stop access to antibody pairs, bulk reagents, and custom sourcing for rare targets, supporting diagnostic manufacturers from early R&D to clinical application. For your ITGBL1 research needs, trust CamelBio to deliver reliable raw materials and technical expertise.