Anti-ITGB8 Rabbit Polyclonal Antibody for WB and ELISA - P26012

Core Product Specifications and Parameters

Biological Background: Integrin beta-8 Function and Localization

• ITGB8 (Integrin beta-8) forms a heterodimer with integrin alpha-V to create the alpha-V:beta-8 receptor, which binds fibronectin via an R-G-D (Arg-Gly-Asp) recognition motif. Related references: PMID:1918072
• The alpha-V:beta-8 integrin plays a key role in TGF-beta-1 activation by mediating the R-G-D-dependent release of mature TGF-beta-1 from its latent complex; this process is especially important on the surface of activated regulatory T-cells (Tregs).
• ITGB8 is required for vasculogenesis, the de novo formation of blood vessels during embryonic development.
• ITGB8 is a transmembrane protein localized to the cell membrane.
• The protein is highly expressed in placenta, kidney, brain, ovary, and uterus, and is transiently expressed in 293 human embryonic kidney cells; it is also found in various transformed cell lines.
• ITGB8 is a glycoprotein with an N-terminal signal peptide, an extracellular EGF-like domain, and a single-pass transmembrane helix; its extracellular region contains multiple disulfide bonds and metal-binding sites for calcium and magnesium.
• The ITGB8 gene undergoes alternative splicing, and the protein has been characterized by 3D-structure determination and proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen (aa 43-320) corresponds to an extracellular region of ITGB8, suggesting potential recognition of the native protein in WB under reducing conditions; verify with an appropriate positive control (e.g., placental or kidney tissue lysate).
• For Western blotting, expect a band at approximately 86 kDa. Using a loading control and validating in the relevant species (human, mouse, rat) is advised.
• In ELISA applications, the antibody may be used to detect recombinant or native ITGB8; coating and detection conditions should be optimized by the end user.
• As a polyclonal antibody, lot-to-lot consistency may vary; batch-specific validation and inclusion of appropriate controls are recommended.
• Given the cross-reactivity with mouse and rat, this antibody is suitable for translational models, but users should confirm target specificity in their experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research laboratories, providing this anti-ITGB8 polyclonal antibody as part of a broader portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material procurement. Whether you are developing assays for cell adhesion, immunology, or vascular biology, CamelBio streamlines access to critical IVD raw materials from concept to clinic.