Anti-ITGB7 Polyclonal Antibody for WB, ELISA - P26010

Core Product Specifications and Parameters

Biological Background: ITGB7 Function and Localization

• ITGB7 encodes integrin beta-7, a single-pass transmembrane glycoprotein that pairs with alpha-4 (CD49d) or alpha-E (CD103) subunits to form heterodimeric adhesion receptors, with alternative splicing generating distinct isoforms.
• The ITGA4/ITGB7 integrin (LPAM-1) directs lymphocyte homing to gut-associated lymphoid tissue (GALT) by binding the mucosal vascular addressin MADCAM1; it also interacts with VCAM1 and the CS-1 region of fibronectin.
  Related references: PMID:10837471 PMID:14608374
• The ITGAE/ITGB7 integrin (HML-1) on intraepithelial lymphocytes functions as a receptor for E-cadherin, facilitating tissue retention.
  Related references: PMID:10837471
• During HIV-1 infection, the virus exploits beta-7 integrin to enter GALT by binding its gp120 protein via an L-D-I tripeptide motif, which is a major trigger of AIDS pathogenesis.
• ITGB7 is expressed on the cell membrane of diverse leukocyte lineages and requires divalent cations (Ca²⁺, Mg²⁺) for ligand binding.
• Post-translational modifications include phosphorylation, N-glycosylation, and disulfide bond formation; the extracellular region contains EGF-like repeats essential for structural integrity.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 140 amino acids, targeting the extracellular domain; the resulting antibody may be suitable for detecting both full-length and truncated isoforms.
• For Western blot, a band near 87 kDa is expected under reducing conditions; optimize loading and detection conditions for human and mouse lysates.
• In ELISA, consider using the antibody as a capture reagent in sandwich assays, and validate specificity with recombinant ITGB7 or integrin heterodimers.
• Cross-reactivity with mouse ITGB7 enables translational studies, but additional epitope mapping may be advisable when using mouse models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined supply chain for IVD raw materials, including validated anti-ITGB7 polyclonal antibodies like this product. Whether you need optimized antibody pairs, bulk ancillary reagents, or rare target sourcing for integrin biology or lymphocyte trafficking studies, our one-stop procurement platform supports your workflow from concept to clinical application. Contact us to discuss how we can accelerate your assay development with reliable, high-quality reagents.