Anti-ITGB5 Rabbit Polyclonal Antibody for WB and ELISA - P18084

Core Product Specifications and Parameters

Biological Background: Integrin beta-5 Function and Localization

• Integrin beta-5 (ITGB5) is the beta subunit of the integrin alpha-V/beta-5 heterodimer, a transmembrane receptor for fibronectin that recognizes the RGD sequence.
• The alpha-V/beta-5 integrin mediates cell adhesion to the extracellular matrix, playing a key role in cell migration, proliferation, and survival.
• ITGB5 also functions as a host cell receptor for adenovirus type C, facilitating viral entry via clathrin-mediated endocytosis.
• The protein localizes primarily to the cell membrane and contains characteristic integrin structural features, including EGF-like domains and a large extracellular region stabilized by disulfide bonds.
• ITGB5 is subject to post-translational modifications such as phosphorylation and glycosylation, which may regulate its activity and expression levels.

Experimental Guidance and Technical Tips

• The antibody targets a recombinant fusion protein spanning amino acids 490-720 of human ITGB5, corresponding to a portion of the extracellular domain; this region is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot (WB), consider using whole-cell lysates from human, mouse, or rat cell lines; validate against known positive controls and include appropriate negative controls to confirm specificity.
• For ELISA, test against recombinant ITGB5 or immobilized fibronectin; optimize coating and detection conditions for the specific assay format.
• Because the immunogen is a recombinant polypeptide, the antibody may recognize both native and denatured conformations; empirical verification in the intended sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop IVD raw-material sourcing platform, providing this anti-ITGB5 polyclonal antibody for researchers and diagnostic developers targeting integrin-mediated cell adhesion and viral entry pathways. As a supply partner, CamelBio can support your projects with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance sourcing rare-target raw materials for IVD assay development.