Anti-ITGB3BP Polyclonal Antibody for WB and ELISA - Q13352

Core Product Specifications and Parameters

Biological Background: ITGB3BP Function and Localization

• ITGB3BP (CENPR, NRIF3) is a multifunctional transcription coregulator that can act as either a coactivator or corepressor, and also functions as a component of the centromere/kinetochore complex.
• Isoform 1 specifically coactivates nuclear receptors for retinoid X (RXRs) and thyroid hormone (TRs) in a ligand-dependent manner, as well as estrogen receptor alpha, but does not coactivate receptors for retinoic acid, vitamin D, progesterone, or glucocorticoids.
• Acts as a transcriptional corepressor through interaction with the NFKB1 NF-kappa-B subunit, likely by interfering with its transactivation domain.
• Induces apoptosis selectively in breast cancer cells via a caspase-2-mediated pathway that involves mitochondrial membrane permeabilization but does not require other caspases.
• May function as an inhibitor of cyclin A-associated kinase activity.
• As a member of the CENPA-CAD (nucleosome distal) complex, it is recruited to centromeres and is essential for kinetochore protein assembly, proper mitotic progression, and chromosome segregation.
• May facilitate the incorporation of newly synthesized CENPA into centromeres by interacting with the CENPA-NAC complex.
• Subcellular localization includes nucleus, centromere, kinetochore, and cytoplasm.
• Widely expressed across tissues, with high expression in testis and colon; isoform 4 is specifically expressed in platelets, lymphocytes, and granulocytes.
• Post-translational modifications include phosphorylation, ubiquitination, and the presence of an isopeptide bond.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1–111) of human ITGB3BP, which is conserved in mouse and rat, consistent with the observed cross-reactivity. Validate performance in your specific model system.
• For Western blotting, the predicted molecular weight is approximately 20 kDa; use appropriate gel percentage (e.g., 12–15% SDS-PAGE) and optimize transfer conditions for small proteins.
• For ELISA applications, consider titrating the coating antigen and antibody to achieve optimal signal-to-noise ratios for your specific assay format.
• As a polyclonal antibody, lot-to-lot variability may occur; we recommend including positive and negative controls in each experiment and validating consistency if switching batches.
• This antibody can be used to study ITGB3BP's roles in transcription regulation, apoptosis, and centromere/kinetochore biology; verify specificity via knockdown or knockout controls when feasible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and services, empowering diagnostic manufacturers and researchers from concept to clinic. This anti-ITGB3BP polyclonal antibody supports investigations into transcriptional regulation and centromere biology, and we can further assist with sourcing validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents tailored to your assay development needs. Connect with us to explore custom sourcing solutions for rare targets and technical consulting.