Anti-ITGB3BP Polyclonal Antibody for IHC-P and ELISA - Q13352

Core Product Specifications and Parameters

Biological Background: ITGB3BP Function and Localization

• ITGB3BP, also known as Centromere protein R (CENPR), Beta-3-endonexin, and Nuclear receptor-interacting factor 3 (NRIF3), is a multifunctional protein encoded by the ITGB3BP gene.
• It acts as a transcription coregulator with both coactivator and corepressor functions, interacting with nuclear receptors such as retinoid X receptors (RXRs) and thyroid hormone receptors (TRs), and modulating NF-kappa-B activity.
• Isoform 1 specifically mediates ligand-dependent coactivation of RXRs and TRs, while not affecting retinoic acid, vitamin D, progesterone, or glucocorticoid receptors.
• Plays a role in apoptosis, inducing caspase-2-mediated cell death in breast cancer cells but not other cancer types, involving mitochondrial membrane permeabilization.
• Serves as a component of the CENPA-CAD complex at centromeres, contributing to kinetochore assembly, mitotic progression, and chromosome segregation; may facilitate incorporation of newly synthesized CENPA into centromeres.
• Subcellular localization includes the nucleus (centromere, kinetochore), cytoplasm, and potentially both compartments, reflecting its dual roles in transcription and chromosome segregation.
• Widely expressed, with high levels in testis and colon; isoform 4 is specifically found in platelets, lymphocytes, and granulocytes.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond), and the protein is subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-111) of human ITGB3BP. This region may detect multiple isoforms; however, isoform-specific expression should be verified in the target sample.
• For IHC-P, optimize antigen retrieval conditions (e.g., heat-induced epitope retrieval at pH 6 or 9) and antibody dilution. Validate staining specificity using appropriate positive and negative tissue controls.
• In ELISA applications, consider using a positive control (e.g., recombinant protein or cell lysate) to confirm binding, and include a blocking peptide competition assay to assess specificity.
• The predicted molecular weight is 20 kDa. If adapting this antibody for western blotting, confirm that the observed band aligns with the expected size and consider the potential for post-translational modifications or isoforms.
• This polyclonal antibody may exhibit species cross-reactivity with mouse and rat, but performance should be empirically validated in the desired species and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a trusted partner, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialize in sourcing rare-target raw materials for centromere biology, transcription regulation, and apoptosis research. Whether you need antibody customization, technical consultation, or procurement support for your diagnostic assays, CamelBio bridges your research to reliable supply.