Anti-ITGA9 Polyclonal Antibody for WB, ELISA - Q13797

Core Product Specifications and Parameters

Biological Background: Integrin alpha-9 Function and Localization

• Integrin alpha-9 (ITGA9), also known as integrin alpha-RLC, is a member of the integrin alpha chain family and pairs with beta-1 integrin to form a functional receptor.
• ITGA9:ITGB1 serves as a receptor for VCAM1, cytotactin, and osteopontin, recognizing the amino acid sequence A-E-I-D-G-I-E-L in cytotactin.
• Through interaction with SVEP1, ITGA9:ITGB1 inhibits vasocontraction by repressing PRKCA-mediated L-type voltage-gated channel Ca²⁺ influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells. Related references: PMID:35802072
• ITGA9 is localized to the cell membrane, consistent with its role in cell adhesion and signal transduction.
• The protein is expressed in vascular smooth muscle cells and the airway epithelium, as confirmed at the protein level. Related references: PMID:35802072, PMID:8245132
• ITGA9 contains typical integrin structural features including disulfide bonds, glycosylation sites, and calcium-binding domains, reflecting its functions in metal-dependent cell adhesion.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 30–250 of human ITGA9, which lies within the extracellular domain; the resulting polyclonal antibody may recognize multiple epitopes.
• For Western blotting, a band near 114 kDa is expected; consider using appropriate loading controls and optimizing blocking conditions.
• This antibody can be used for ELISA applications; titration and pairing with appropriate detection reagents are recommended.
• Cross-reactivity with mouse has been observed; validation in the relevant sample system (e.g., mouse vascular or epithelial tissue) is advised.
• Given the role of ITGA9 in cell adhesion and vascular function, the antibody may be useful in cell adhesion assays or functional studies, though these applications have not been validated by the supplier.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with reliable access to a comprehensive portfolio of IVD raw materials. For studies on integrins, cell adhesion, or vascular biology, we can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target sourcing. Our integrated services help bridge the gap from concept to clinic, ensuring you receive consistent, high-quality materials for your assay development.