Anti-ITGA8 Rabbit Polyclonal Antibody for WB and ELISA - P53708

Core Product Specifications and Parameters

Biological Background: Integrin alpha-8 Function and Localization

• Integrin alpha-8 (ITGA8) is a single-pass type I membrane protein that belongs to the integrin alpha chain family. It heterodimerizes with integrin beta-1 to form a receptor for extracellular matrix proteins.
• The alpha-8/beta-1 integrin recognizes the RGD motif in multiple ligands, including tenascin-C (TNC), fibronectin (FN1), osteopontin (SPP1), TGFB1, TGFB3, and vitronectin (VTN), mediating cell adhesion and signaling.
• ITGA8 plays a critical role in kidney development by regulating the recruitment of mesenchymal cells into epithelial structures; nephronectin (NPNT) is likely its functional ligand in kidney genesis.
• In the nervous system, ITGA8 serves as a neuronal receptor for tenascin-C, contributing to cell-cell interactions and regulating neurite outgrowth of sensory and motor neurons.
• Expressed predominantly in mesenchymal cells, including alveolar myofibroblasts, kidney mesangial cells, hepatic stellate cells, and vascular and visceral smooth muscle.
• Subcellular localization: Cell membrane; single-pass transmembrane protein with a large extracellular domain, a transmembrane helix, and a short cytoplasmic tail.
• Contains characteristic integrin structural repeats and is subject to post-translational modifications including cleavage on pair of basic residues, disulfide bond formation, and glycosylation.

Experimental Guidance and Technical Tips

• Immunogen Design: Raised against a recombinant fragment covering amino acids 907–1000 (cytoplasmic domain) of human ITGA8. This region is not typically shed, so the antibody is expected to recognize the full-length membrane-bound form in cell lysates.
• Western Blot: A specific band at ~117 kDa under reducing conditions is anticipated. Use whole cell or tissue lysates from kidney, smooth muscle, or mesenchymal cell lines as positive control material. Validate performance in human, mouse, and rat samples due to reported cross-reactivity.
• ELISA: The polyclonal antibody can be employed as a capture or detection reagent in indirect or sandwich ELISA formats. Optimize coating concentration (e.g., 1–10 µg/mL) and blocking conditions. Pairing with a monoclonal anti-ITGA8 may improve specificity in sandwich assays.
• General Tip: Because the antibody recognizes an intracellular epitope, it is not recommended for detection of soluble or shed extracellular ITGA8 fragments. For applications requiring detection of native surface protein, consider alternative antibodies targeting the extracellular domain.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to high-quality IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. For research focused on kidney development, cell adhesion, or neurogenesis, this ITGA8 polyclonal antibody is part of our extensive catalog of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Additionally, our team can assist with rare target raw-material sourcing and custom assay development to accelerate your diagnostic projects.