Anti-ITGA1/CD49a Monoclonal Antibody () for FC - P56199

Core Product Specifications and Parameters

Biological Background: ITGA1/CD49a Function and Localization

• Protein identity and isoforms: Integrin alpha-1 (ITGA1), also known as CD49a, VLA-1 alpha subunit, or CD49 antigen-like family member A, is a single-pass type I transmembrane glycoprotein encoded by the ITGA1 gene.
• Heterodimer formation: It non-covalently associates with integrin beta-1 (CD29) to form the functional laminin and collagen receptor VLA-1 (very late antigen-1).
• Ligand specificity: The integrin alpha-1/beta-1 complex recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen and also binds laminin.
• Functional role: ITGA1 mediates anchorage-dependent, negative regulation of EGF-stimulated cell growth, contributing to cell adhesion and signaling processes.
• Subcellular localization: The protein is located in the cell membrane, with a large extracellular domain, a single transmembrane helix, and a short cytoplasmic tail.
• Post-translational modifications and domains: ITGA1 contains multiple calcium- and magnesium-binding domains, disulfide bonds, and is heavily glycosylated. It includes an N-terminal signal peptide and multiple repeat regions.
• Molecular weight: The mature protein has a calculated molecular weight of approximately 130,848 Da, consistent with the reported ~131 kDa band observed under reducing conditions.

Experimental Guidance and Technical Tips

• The immunogen spans the large extracellular domain (amino acids 29–1141), suggesting the antibody binds an extracellular epitope accessible for live-cell staining in flow cytometry without the need for permeabilization.
• Since the antibody is directly conjugated to , no secondary detection reagent is required, streamlining the FC workflow.
• Consider titrating the antibody (e.g., 0.1–1 µg per test) on the target cell type to determine the optimal staining concentration. Use an appropriate isotype control conjugated to the same fluorophore to assess non-specific binding.
• Validate antibody performance in human samples expressing ITGA1/CD49a, such as activated T cells, endothelial cells, or certain epithelial cell lines.
• For cell surface staining, use standard live-cell protocols; avoid fixation unless necessary, as it may alter epitope accessibility or fluorophore brightness.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-ITGA1/CD49a monoclonal antibody, detecting a critical cell adhesion receptor, is available along with a full portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your immunoassay development. For rare targets or custom requirements, CamelBio’s global sourcing network ensures streamlined procurement, helping you accelerate your IVD projects with confidence.