Anti-ITGA10 Rabbit Polyclonal Antibody for WB and ELISA - O75578

Core Product Specifications and Parameters

Biological Background: ITGA10 Function and Localization

• ITGA10 encodes integrin alpha-10, a transmembrane receptor subunit that pairs with integrin beta-1 to form a functional collagen receptor.
• The integrin alpha-10/beta-1 heterodimer specifically binds collagen, mediating cell-matrix adhesion and signaling pathways critical for tissue integrity and development.
• ITGA10 is widely expressed, with highest levels in muscle and heart, and is notably found in articular cartilage, suggesting roles in musculoskeletal maintenance.
• The protein localizes to the plasma membrane as a single-pass type I membrane protein.
• Keywords indicate ITGA10 undergoes alternative splicing, is a glycoprotein with disulfide bonds, and binds calcium and magnesium, which are essential for its adhesive functions.
• UniProt annotates its molecular weight at ~127.6 kDa, with a calculated mass of 127,602 Da.

Experimental Guidance and Technical Tips

• The immunogen targets the C-terminal cytoplasmic region (residues 801-1040), which is specific to the intracellular domain of ITGA10; this design may enhance isoform specificity.
• For Western blotting, expect a band around 128 kDa; consider using tissue lysates from muscle, heart, or cartilage as positive controls and validate with appropriate loading controls.
• For ELISA, consider coating with recombinant full-length ITGA10 or its extracellular domain to capture the antibody; perform titration to determine optimal working concentrations.
• Cross-reactivity with mouse suggests conservation of the epitope sequence; verify reactivity in relevant mouse tissue samples, such as skeletal muscle or cartilage extracts.
• Note that polyclonal antibodies may exhibit lot-to-lot variability; it is recommended to validate each new lot in your specific experimental system.

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CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research labs, providing reliable access to raw materials like this ITGA10 polyclonal antibody for collagen receptor studies. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, accelerating your assay development from concept to clinic.