Anti-ITFG1 Polyclonal Antibody for WB, ELISA - Q8TB96

Core Product Specifications and Parameters

Biological Background: ITFG1 Function and Localization

• ITFG1 (Integrin-alpha FG-GAP repeat-containing protein 1), also known as TIP, CDA08, or Linkin, is encoded by the ITFG1 gene and functions as a modulator of T-cell activity, with a protective role in graft-versus-host disease models.
• The protein contains FG-GAP repeats, characteristic of integrins, suggesting potential roles in cell adhesion or signaling.
• ITFG1 is classified as a secreted and membrane-associated glycoprotein with a signal peptide and a transmembrane helix, indicating its presence at the cell surface and in extracellular spaces.
• It is ubiquitously expressed across human tissues, reflecting its fundamental role in immune regulation.
• As a glycoprotein, ITFG1 may undergo post-translational modifications critical for its function and localization.
• The predicted molecular weight of ITFG1 is approximately 68 kDa, consistent with observations in immunoblotting under appropriate conditions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 313-612) of human ITFG1, overlapping with predicted functional domains; this may help generate antibodies recognizing native epitopes.
• For Western blotting, a band around 68 kDa is expected in cell lysates from human, mouse, and rat tissues; validate with appropriate positive and negative controls.
• In ELISA applications, the polyclonal nature offers broad epitope coverage; however, lot-to-lot consistency should be confirmed for quantitative assays.
• Consider antigen retrieval or native conditions if membrane localization masks epitopes in fixed samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering this high-quality anti-ITFG1 polyclonal antibody alongside complementary reagents for T-cell immunology and transplantation research. From validated antibody pairs to bulk ancillary reagents, we bridge the gap between concept and clinic for diagnostic manufacturers and laboratories worldwide.