Anti-ITCH Rabbit Monoclonal Antibody (KO Validated) for WB, ELISA - Q96J02

Core Product Specifications and Parameters

Biological Background: ITCH Function and Localization

• ITCH (E3 ubiquitin-protein ligase Itchy homolog), also known as AIP4, NAPP1, is a HECT-type E3 ubiquitin ligase.
• Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers it to targeted substrates. Related references: PMID:11046148, PMID:14602072, PMID:15051726
• Catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation, enabling diverse signaling outcomes. Related references: PMID:17028573, PMID:18718448, PMID:19131965
• Essential component of a ubiquitin-editing protein complex (A20 complex) that ensures the transient nature of inflammatory signaling by promoting RIPK1 degradation. Related references: PMID:19131965
• Negatively regulates MAVS-dependent antiviral responses by ubiquitinating MAVS through 'Lys-48'-linked conjugation, leading to its proteasomal degradation. Related references: PMID:19881509
• Regulates endosomal sorting and degradation of receptors such as CXCR4 and FZD4 via ubiquitination of ESCRT-0 components and PI4K2A. Related references: PMID:14602072, PMID:23146885, PMID:34927784
• Together with UBR5, regulates apoptosis and reactive oxygen species levels through 'Lys-48'-/'Lys-63'-branched ubiquitination and proteasomal degradation of TXNIP. Related references: PMID:20068034, PMID:29378950
• Localizes to the cell membrane, cytoplasm, nucleus, and endosome membranes.
• Widely expressed across tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–100 of human ITCH, so the antibody likely recognizes the N-terminal region. Confirm specificity using knockout cell lysates or peptide competition assays.
• For WB, the predicted molecular weight is 103 kDa. Optimize starting dilution using relevant cell lysates (e.g., Jurkat, HeLa) from human, mouse, or rat origin.
• The antibody is listed as KO validated; consider using ITCH knockout cell lines to verify specificity in your experimental system.
• For ELISA, validate coating concentrations and detection antibody conditions with recombinant ITCH protein or known positive samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibodies like this ITCH monoclonal antibody. We supply bulk monoclonal and polyclonal antibodies, antibody pairs, and ancillary reagents to streamline assay development. For challenging targets in ubiquitination and immune signaling pathways, our team provides reliable sourcing and technical support from concept to clinic.