Anti-ITCH Polyclonal Antibody for WB, IHC-P, ELISA - Q96J02

Core Product Specifications and Parameters

Biological Background: ITCH Function and Localization

• ITCH (E3 ubiquitin-protein ligase Itchy homolog, also known as AIP4, AIF4, ADMFD, NAPP1) is a HECT domain-containing E3 ligase that accepts ubiquitin from E2 enzymes and directly transfers it to substrate proteins. Related references: PMID:11046148, PMID:14602072, PMID:15051726
• It catalyzes Lys-29-, Lys-48-, and Lys-63-linked polyubiquitin conjugation, critical for controlling inflammatory signaling pathways; as part of a ubiquitin-editing complex with TNFAIP3, TAX1BP1, and RNF11, ITCH ensures transient NF-kappa-B activation by mediating RIPK1 degradation. Related references: PMID:17028573, PMID:18718448, PMID:19131965
• ITCH regulates the transcriptional activity of multiple factors (e.g., JUN, JUNB, NFE2) and immune response molecules (e.g., MAVS, RIPK2) through ubiquitination, influencing Th2 cytokine production, hematopoietic development, and antiviral signaling. Related references: PMID:16387660, PMID:18718448, PMID:19592251, PMID:19881509
• Localized to the cell membrane, cytoplasm, nucleus, and endosomal membranes, ITCH mediates receptor sorting (e.g., CXCR4, FZD4) by ubiquitinating ESCRT-0 components and PI4K2A, linking ubiquitination to endocytic trafficking. Related references: PMID:14602072, PMID:23146885, PMID:34927784
• ITCH promotes apoptosis and redox balance by targeting TXNIP for branched ubiquitination together with UBR5, and mediates EGF antiapoptotic signaling via p15 BID degradation. Related references: PMID:20068034, PMID:20392206, PMID:29378950
• It inhibits influenza A virus replication by catalyzing Lys-48-linked ubiquitination and proteasomal degradation of the viral M1 protein. Related references: PMID:30328013
• ITCH is widely expressed and subject to acetylation, phosphorylation, and alternative splicing, with a molecular weight of approximately 103 kDa.

Experimental Guidance and Technical Tips

• Given the immunogen corresponds to the N-terminal region (aa 1-155) of human ITCH, this polyclonal antibody is likely to detect full-length ITCH (~103 kDa) as well as potential isoforms in human, mouse, and rat lysates.
• For WB, consider reducing conditions and validate band specificity using positive control cell lines (e.g., Jurkat, HeLa) and a negative control (siRNA knockdown).
• For IHC-P, optimize antigen retrieval methods and antibody dilution using tissue sections known to express ITCH.
• In ELISA, test direct or capture formats and confirm reactivity with recombinant ITCH protein.
• Always include appropriate controls tailored to your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio serves as your strategic one-stop sourcing partner for IVD raw materials, providing validated antibodies, optimized reagents, and technical support from concept to clinic.
• This anti-ITCH polyclonal antibody is part of our extensive portfolio for ubiquitination and immune signaling research; we also offer antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets.
• Partner with us to streamline your procurement and accelerate diagnostic development.