Anti-ISYNA1 Rabbit Polyclonal Antibody for WB and ELISA - Q9NPH2

Core Product Specifications and Parameters

Biological Background: ISYNA1 Function and Localization

• ISYNA1, also known as inositol-3-phosphate synthase 1, myo-inositol 1-phosphate synthase (MIP synthase), or hINO1, is the key enzyme in the myo-inositol biosynthesis pathway.
• Catalyzes the NAD-dependent conversion of glucose 6-phosphate to 1-myo-inositol 1-phosphate, a critical step for producing inositol-containing compounds (PubMed:15024000, PubMed:23902760).
  Related references:
  PMID:15024000
  PMID:23902760
• Acts as the rate-limiting enzyme in the synthesis of all inositol-containing molecules, including phosphoinositides and inositol phosphates, thus influencing diverse cellular processes (PubMed:15024000).
  Related references:
  PMID:15024000
• Localizes primarily to the cytoplasm where it exerts its catalytic function.
• Highly expressed in testis, ovary, heart, placenta, and pancreas, with weaker expression in blood leukocytes, thymus, skeletal muscle, and colon, reflecting tissue-specific demands for inositol.
• The protein undergoes alternative splicing, producing multiple isoforms, and is regulated by phosphorylation; it is classified under isomerases and lipid biosynthesis enzymes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–250) of human ISYNA1; consider this when interpreting isoform-specific detection.
• For Western blot (WB), the predicted molecular weight is approximately 61 kDa; variations may arise from post-translational modifications or alternative splicing.
• Human, mouse, and rat reactivity has been indicated; verify cross-reactivity in your specific experimental model by sequence alignment.
• When performing ELISA, titrate the antibody and use appropriate blocking buffers to minimize nonspecific binding.
• Include positive and negative controls from tissues with high and low ISYNA1 expression (e.g., testis vs. leukocytes) to validate specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing ready-to-use IVD raw materials like this anti-ISYNA1 antibody, catering to studies in inositol metabolism and lipid signaling. Our comprehensive portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Rely on CamelBio as your efficient bridge from concept to clinical application.