Anti-ISY1 Polyclonal Antibody for WB, ELISA - Q9ULR0

Core Product Specifications and Parameters

Biological Background: ISY1 Function and Localization

• Pre-mRNA-splicing factor ISY1 homolog is a subunit of the spliceosome C complex, essential for pre-mRNA splicing.
• It regulates microRNA (miRNA) biogenesis, selectively processing miRNAs from primary transcripts such as pri-miR-17-92, pri-miR-290-295, and pri-miR-96-183 (By similarity).
• ISY1 functions in embryonic stem cell (ESC) differentiation, promoting the exit from naive pluripotency to a primed state via enhanced miRNA production (By similarity).
• The protein localizes to the nucleus, consistent with its role in mRNA processing.
• Post-translational modifications including acetylation and phosphorylation suggest regulatory control of ISY1 activity.
• Alternative splicing of the ISY1 gene produces multiple isoforms, potentially diversifying its function.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1-307 of human ISY1 (NP_001186398.1), which may detect multiple isoforms of the protein.
• For Western blot, consider testing human and mouse cell lysates to confirm species cross-reactivity; a band around 33 kDa is expected.
• ELISA optimization can be performed using recombinant ISY1 protein as a coating antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this anti-ISY1 polyclonal antibody as part of a comprehensive IVD raw-material portfolio, serving diagnostic manufacturers and research laboratories. We offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, supporting your miRNA and splicing research programs from concept to clinic.